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Preparation technology and application of high-protein-content high-nucleic-acid-content yeast hydrolyate

A nucleic acid yeast and preparation process technology, applied in the application, animal feed, animal feed and other directions, can solve the problem of not using feed additives for lactating sows, and achieve the effect of improving the ability of reproduction and improving the level of lactation.

Active Publication Date: 2017-09-01
广东雅琪生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glutathione is a tripeptide that can help maintain normal immune system functions, and has antioxidant and integrated detoxification effects. It can also be used as a base material for functional foods to delay aging, enhance immunity, and Functional foods such as tumors are widely used, but are not currently used as feed additives for lactating sows

Method used

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Examples

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Effect test

preparation example Construction

[0027] The preparation process of high-protein and high-nucleic acid yeast hydrolyzate comprises the following steps:

[0028] (1) Inoculate the yeast into the fermentation medium, and ferment at 28-35°C until the biomass in the fermentation liquid is 50-70g / L;

[0029] (2) After the yeast obtained by fermentation is centrifuged and washed, it is concentrated to obtain a fermentation broth containing 100-150 g / L of yeast dry matter;

[0030] (3) Adjust the pH value of the fermentation broth to 4.5-6.5, and perform ultrasonic treatment for 40-60 minutes under the condition that the ultrasonic power is 400-600W and the temperature is 30-40°C;

[0031] (4) After stopping the ultrasonic treatment, keep the temperature at 45°C to 55°C for 6 to 8 hours, then raise the temperature to 100°C and boil for 10 to 30 minutes to obtain the autolyzed yeast liquid;

[0032] (5) After the temperature of the autolyzed yeast liquid drops to 55°C-60°C, adjust the pH value to 5.5-6.5, add 0.05-0....

Embodiment 1

[0046]Embodiment 1, preparation process 1 of high protein and high nucleic acid yeast hydrolyzate

[0047] The preparation process 1 of high-protein and high-nucleic acid yeast hydrolyzate comprises the following steps:

[0048] 1) Slant-plane strain: inoculate yeast into solid slant medium, culture at 28°C for 20 hours, and obtain slant-plane yeast strain;

[0049] 2) Primary liquid seed culture: Take 2-3 rings of slanted yeast strains into 200mL liquid seed medium, culture at 28°C, 200rpm for 20h, and obtain primary seeds;

[0050] 3) Secondary liquid seed culture: put the first-grade seeds into a fermenter equipped with 10L liquid seed culture medium according to the inoculation amount of 2%, and cultivate them with stirring at 28°C for 20 hours to obtain the second-grade seeds;

[0051] 4) Fermentation tank fermentation: the secondary seed culture liquid was inserted into a fermenter tank equipped with 100L liquid fermentation medium according to the inoculum size of 10%,...

Embodiment 2

[0058] Embodiment 2, preparation process 2 of high protein and high nucleic acid yeast hydrolyzate

[0059] The preparation process 2 of high-protein and high-nucleic acid yeast hydrolyzate comprises the following steps:

[0060] 1) Slant-plane strain: inoculate yeast into solid slant medium, culture at 28°C for 20 hours, and obtain slant-plane yeast strain;

[0061] 2) Primary liquid seed culture: Take 2-3 rings of slanted yeast strains into 200mL liquid seed medium, culture at 28°C, 200rpm for 20h, and obtain primary seeds;

[0062] 3) Secondary liquid seed culture: put the first-grade seeds into a fermenter equipped with 10L liquid seed culture medium according to the inoculation amount of 2%, and cultivate them with stirring at 28°C for 20 hours to obtain the second-grade seeds;

[0063] 4) Fermentation tank fermentation: the secondary seed culture liquid was inserted into a fermenter tank equipped with 100L liquid fermentation medium according to the inoculum size of 10%...

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Abstract

The invention discloses a preparation technology and application of high-protein-content high-nucleic-acid-content yeast hydrolyate. The preparation technology of the high-protein-content high-nucleic-acid-content yeast hydrolyate comprises the following steps of performing high-density liquid fermentation on yeast strains, performing washing, performing ultrasonic treatment, performing autolysis, performing enzymolysis, performing concentration, performing spray drying and the like, so that the high-protein-content high-nucleic-acid-content yeast hydrolyate is obtained, wherein the crude protein content is greater than or equal to 50wt%, the nucleic acid content is greater than or equal to 10wt%, the yeast cell wall polysaccharide is greater than or equal to 15wt%, the ash content is smaller than or equal to 6wt%, and the purity is high. A feed additive for lactation sows is prepared in a manner that the prepared high-protein-content high-nucleic-acid-content yeast hydrolyate, glutathione and chitosan oligosaccharide and a carrier are matched and reasonably compounded, and a certain amount of the feed additive is added to a ration, so that the milk secretion level of the lactation sows can be increased, and the repopulation capacity of the lactation sows can be improved.

Description

technical field [0001] The invention belongs to the field of feed additives, and more specifically relates to the preparation process and application of high-protein and high-nucleic acid yeast hydrolyzate. Background technique [0002] At present, the key to restricting the development of the pig industry is the low production capacity and reproductive capacity of sows. Breast milk is the main source of nutrients for piglets from birth to weaning, and the quality and quantity of breast milk determine the survival rate and growth rate of piglets. The low lactation ability of sows can directly lead to nutritional deficiencies, growth retardation, and increased mortality in piglets, affect piglets' weaning weight, aggravate piglets' weaning stress, and affect subsequent growth and development. Therefore, the basic goal of the sow during lactation is to ensure that the sow takes in enough nutrients, secretes enough milk, and maintains a proper body condition, so that the sow c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/147A23K20/163A23K10/30A23K10/37A23K50/30A23K10/18A23K20/10
CPCA23K10/18A23K10/30A23K10/37A23K20/10A23K20/147A23K20/163A23K50/30Y02P60/87
Inventor 董爱华陈训银梁红冯业李彪
Owner 广东雅琪生物科技股份有限公司
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