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Molecular marker hsa-circ-0000705 for gastric cancer and application of molecular marker hsa-circ-0000705

A molecular marker and gastric cancer technology, applied in the field of tumor markers, can solve the problems of unsatisfactory sensitivity and specificity of tumor markers, achieve good gastric cancer tissue specificity and gastric cancer correlation, and improve the level of diagnosis and treatment

Inactive Publication Date: 2017-08-22
邵永富
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Problems solved by technology

Unfortunately, even with the most advanced clinical physical detection methods (such as: CT, MRI, etc.), the limit diameter of tumors is only 1cm, and the sensitivity and specificity of commonly used tumor markers are not ideal, such as carcinoembryonic The sensitivity and specificity of gastric cancer screening by antigen (carcinoembryonic antigen, CEA) are only 36.0% and 86.2% respectively

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  • Molecular marker hsa-circ-0000705 for gastric cancer and application of molecular marker hsa-circ-0000705
  • Molecular marker hsa-circ-0000705 for gastric cancer and application of molecular marker hsa-circ-0000705
  • Molecular marker hsa-circ-0000705 for gastric cancer and application of molecular marker hsa-circ-0000705

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Embodiment 1

[0028] (1) Sample collection and processing

[0029] Surgery is performed on patients with gastric cancer to remove diseased tissue. Cut out a piece of soybean-like tissue from the gastric cancer lesion and 3 cm away from the lesion, cut it into a small piece of tissue with a thickness of no more than 0.5 cm with a scalpel, and soak it in 1 mL RNAfixer non-liquid nitrogen sample RNA preservation solution (Beijing Biotech). Put it in a -80°C ultra-low temperature freezer for later use.

[0030] (2) Design and synthesis of specific PCR reverse primers

[0031] With the help of PrimerPremier primer design software, a specific PCR reverse primer (divergent primer) for the detection of hsa_circ_0000705 was designed independently, and Shanghai Sangon Bioengineering Co., Ltd. was commissioned to synthesize it. The upstream primer sequence of the primer is 5'-TAACTGGGGCTCTTCAGCTC-3', and the downstream primer sequence is 5'-TGGTGGTTGTCTGGCCTTAT-3'. By sequencing the PCR products of...

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Abstract

The invention relates to a molecular marker hsa-circ-0000705 for a gastric cancer and an application of the molecular marker hsa-circ-0000705. A nucleotide sequence is as shown in SEQ ID NO.1. The molecular marker has excellent gastric cancer tissue specificity and gastric cancer relevance; quantitative determination can be achieved through an RT-PCR method; a novel molecular marker is provided for discovery and detection of an early gastric cancer; and the molecular marker has certain significance in further improvement of the current gastric cancer diagnosis and treatment level.

Description

technical field [0001] The invention belongs to the field of tumor markers, in particular to a gastric cancer molecular marker hsa_circ_0000705 and its application. Background technique [0002] With the widespread application of high-throughput genome-wide sequencing technology, it was surprisingly found that about 98% of transcripts in mammalian cells are noncoding RNA molecules (noncoding RNA, ncRNA). These non-coding RNA molecules include the well-known "housekeeping" non-coding RNAs (such as transfer RNAs and ribosomal RNAs, etc.), small non-coding RNAs (such as microRNAs and piRNAs, etc.), long non-coding RNAs RNA (long noncoding RNA, lncRNA), and more circular RNA (circular RNA, circRNA) molecules that have yet to be further studied. [0003] CircRNA refers to a type of endogenous circular RNA molecule produced by alternative splicing of precursor RNA and bound by reverse covalent bonds at the 5' end and 3' end. Preliminary research results at home and abroad show t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/112C12Q2600/158
Inventor 邵永富叶国良郭俊明陆佳敏
Owner 邵永富
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