Cellulose degrading arthrobacterium HW-17 strain and application thereof

Abstract

The invention discloses a high-yield cellulase bacterium which is screened and separated from rotten wood of old-growth forests in Tujia and Miao nationality municipalities in Enshi by using an orient domestication method with a selective medium, the strain is named as HW-17, belongs to arthrobacterium and is preserved in the China Center for Type Culture Collection in Wuhan University in Wuhan, China, with the zip code being 430072, the preservation date being March 30, 2017, and the preservation number being CCTCC NO: M2017156; the 16S rDNA (Deoxyribonucleic Acid) sequence of the strain is as shown in SEQ ID NO.1. The HW-17 strain disclosed by the invention can be applied to industrial production of organic fertilizers with chicken manure or pig manure, crop straw, rotten vegetable leaves and the like as raw materials. In addition, the growth properties and the cellulase production properties of the HW-17 strain are very clear, so the HW-17 strain can be used as a candidate strain for industrial production of cellulase, and commercial production of single cellulase as well.

Description

technical field [0001] The invention relates to a new Arthrobacter strain capable of degrading cellulose, in particular to the use of the strain in producing farmyard manure with agricultural and aquaculture waste as raw materials, and belongs to the technical field of microorganisms. Background technique [0002] Crop straws such as rice straw, wheat straw, bagasse and other cellulose-rich crops are an important renewable resource. The annual output of straw in the world is about 2.2 billion tons (Kim et al., 2004), and my country produces about 2.2 billion tons of straw every year. 700 million tons (Xu Jie et al., 2008). Except for a small part of crop straws used for composting and livestock feed, most of them are burned randomly, which not only wastes precious resources, but also pollutes the environment (Cheng et al., 2013). At present, microbial degradation of crop straw has attracted widespread attention due to its most economical and effective advantages (Gu Wenjie e...

AI Technical Summary

Problems solved by technology

In the practical application of microbial degradation of cellulose, the way of cellulose degrading mixed bacterial flora is often used to degrade the cellulose in crop straw in the form of compost or retting (Liang Dongli et al., 2009). The process period is long and the degradation is slow. full

In recent years, although bioengineering techniques such as artificial mutagenesis and genetic modification (Fujii et al., 2013) have been gradually applied to the transformation of cellulose-degrading bacteria to improve the cellulase-producing ability of the strains, the obtained strains still have enzyme-producing Small amount, low enzyme activity and other problems