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Type 4 bluetongue virus vp2 protein neutralizing B cell antigen epitope polypeptide and application thereof

A bluetongue virus and antigen epitope technology, applied in the field of medicine, can solve problems such as high mortality, acute onset, and damage to the breeding industry

Active Publication Date: 2020-02-11
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, type 4 BTV can infect ruminants such as sheep and camels, with acute onset and high mortality, causing huge damage to the breeding industry

Method used

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  • Type 4 bluetongue virus vp2 protein neutralizing B cell antigen epitope polypeptide and application thereof
  • Type 4 bluetongue virus vp2 protein neutralizing B cell antigen epitope polypeptide and application thereof
  • Type 4 bluetongue virus vp2 protein neutralizing B cell antigen epitope polypeptide and application thereof

Examples

Experimental program
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Embodiment 14

[0019] Example 1 Identification of Neutralizing B Cell Antigen Epitope of Type 4 Bluetongue Virus VP2 Protein

[0020] 1 Materials and methods

[0021] 1.1 Cells and main reagents

[0022] Anti-VP2 protein MAb 1G7 cell line is preserved by our laboratory. HRP-labeled goat anti-mouse IgG was purchased from Zhongshan Jinqiao Co., Ltd.; 1-24 BTV standard positive serum and negative serum were purchased from Pirbright Institute, a bluetongue reference laboratory; Ph.D.-12TM13 phage peptide library kit was purchased from New York, USA England Biolabs (NEB).

[0023] 1.2 Gene sequence

[0024] See Table 1 for the GenBank accession numbers of BTV 1-24 types used for comparison.

[0025] Table 1 GenBank accession numbers of BTV 1-24 types

[0026]

[0027]

[0028] 1.3 Antigen epitope screening

[0029] Referring to the instructions of the Ph.D.-12TM13 phage peptide library, the purified MAb ascites was used as the antigen, added to a microwell plate, and the phage peptide...

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Abstract

The invention discloses a 4-type bluetongue virus (BTV) VP2 protein neutralizing B cell epitope polypeptide and its application. The BTV VP2 protein epitope amino acids are screened by a phage display technology. After three rounds of screening, a sequencing process is carried out. Through analysis and comparison, a sequencing result shows that the common short peptide sequence is 160NH161. Experiment results show that 160NH161 can specifically react with the supernatant and ascites of 4A-1G7 and has good reaction specificity to 1 to 24-type BTV positive standard serum. The 4-type BTV VP2 protein neutralizing B cell epitope polypeptide provides a novel technical process for 4-type BTV clinical diagnosis and lays a basis for later building of a BTV-type specific identification and diagnosis method and preparation of a 4-type BTV related epitope vaccine.

Description

technical field [0001] The present invention relates to an antigenic epitope polypeptide and its application, in particular to a neutralizing B cell antigenic epitope polypeptide of type 4 bluetongue virus VP2 protein and its application in diagnosing or treating bluetongue disease. The present invention belongs to medicine technology field. Background technique [0002] Bluetongue (Bluetongue, BT) is an arboviral zoonotic disease common in tropical and subtropical countries that infects ruminants. BT is caused by Bluetongue Virus (BTV), an infectious disease that can infect ruminants such as sheep, cattle and camels. BTV infects animals, plants, insects and is transmitted by arthropods such as mosquitoes, Culicoides, ticks, etc. BTV belongs to the genus Orbivirus, one of twelve genera in the family Reoviridae. BTV was first discovered in 1876 and has a worldwide distribution. At present, BTV has been isolated from many tropical, subtropical and temperate countries in Af...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/14C12N15/46G01N33/68G01N33/569A61K39/15A61P31/14
CPCA61K39/00C07K14/005C12N2720/12122C12N2720/12131C12N2720/12134G01N33/56983G01N33/68
Inventor 唐丽杰李一经徐义刚王丽乔薪瑗姜艳平崔文李佳璇
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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