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Improved anti-nuclear antibody detection and diagnostics for systemic and non-systemic autoimmune disorders

A technology for antibody and antibody detection, which is applied in the direction of introducing foreign genetic material, measuring devices, instruments, etc. by using carriers, and can solve unsatisfied problems

Active Publication Date: 2017-08-18
IMMCO DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Accordingly, there is a continuing and unmet need for improved compositions and methods of detecting ANA

Method used

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  • Improved anti-nuclear antibody detection and diagnostics for systemic and non-systemic autoimmune disorders

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Embodiment Construction

[0026] The present disclosure provides compositions and methods for using the compositions to detect ANA autoantibodies, and / or methods for determining whether a biological sample obtained from or derived from an individual includes antibodies that recognize LEDGF protein, and using A method for diagnosing and / or assisting in diagnosing autoimmune disorders positively correlated with the presence of ANA autoantibodies. Such disorders include, but are not necessarily limited to, systemic autoimmune rheumatic diseases (SARDs). Also provided are kits / products comprising reagents for use in detecting ANA autoantibodies.

[0027] In general, the present disclosure provides methods for reducing and / or eliminating the DFS pattern that often characterizes IIF analysis of ANAs, which in current assays typically relies on HEp-2 cells as a substrate, while including relative In vitro compositions of modified mammalian cells with less LEDGF expression in unmodified HEp-2 cells and method...

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Abstract

Provided are compositions that contain mammalian cells for use in detecting antibodies. The mammalian cells are modified such that they do not contain LEDGF protein. The mammalian cells are immobilized on a solid substrate. The compositions can also contain mammalian cells that contain the LEDGF protein. Methods for using the cell compositions in diagnostic approaches are included, such as kits for performing diagnostic tests.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application No. 61 / 969,771, filed March 24, 2014, the disclosure of which is incorporated herein by reference. technical field [0003] The present invention relates generally to autoimmune diseases, and more particularly to compositions and methods for use in detecting antinuclear antibodies. Background technique [0004] Diagnosing autoimmune diseases is often challenging, and individuals with one autoimmune disease often have a higher risk of developing others, such as systemic autoimmune rheumatic disease (SARD). The presence of antinuclear autoantibodies (ANA) is considered a hallmark of SARD, and this association has been known for some time. The American College of Rheumatology (ACR) recommends testing for ANA by indirect immunofluorescence (IIF) assay using HEp-2 cells, as described in their 2009 position statement. The statement states that HEp-2 cells ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/475
CPCG01N33/5005G01N33/564C12N15/85G01N33/6854
Inventor 拉克什曼阿曼·苏曼什基肖尔·马利阿万泰曼
Owner IMMCO DIAGNOSTICS
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