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Coated oncolytic adenoviruses for cancer vaccines

An adenovirus and virus technology, applied in the field of adenovirus vectors, can solve the problems of laborious adenovirus therapy, slowness, and adenovirus therapy not suitable for personalized medicine

Pending Publication Date: 2017-08-18
瓦洛治疗公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One object is to provide adenoviral vectors with a modified viral surface, their use, and methods of treating disease by stimulating a peptide-specific (i.e., anti-peptide) immune response to address, for example, inefficient, slow, expensive and laborious Adenoviral therapy and its inappropriateness for personalized medicine, among other issues

Method used

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  • Coated oncolytic adenoviruses for cancer vaccines
  • Coated oncolytic adenoviruses for cancer vaccines
  • Coated oncolytic adenoviruses for cancer vaccines

Examples

Experimental program
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Embodiment Construction

[0102] The following examples at least demonstrate the analysis of the tumor MHC-I immunopeptidome used to isolate and select tumor-specific polypeptides, the generation and physical characterization of tumor-specific polypeptide-coated oncolytic adenoviruses, and the expression of coated adenoviruses in animals. Characterization in the model (eg i) therapeutic efficacy, ii) ability to transfer antiviral immunity to antitumor immunity and iii) ability to recruit cells of the immune system and promote T cell responses).

[0103] Oncolytic adenovirus preparation

[0104] All oncolytic adenoviruses (OAd) were generated and propagated using standard protocols as previously described (8). Briefly, virus was amplified by infecting 10T175 flasks with 70-80% confluent A549 cells at a multiplicity of infection (MOI) of 30. Three days after infection, cells were harvested and lysed by four cycles of freezing (-80°C) and thawing (37°C). Adenoviral particles were then separated from cel...

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PUM

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Abstract

The present invention relates to adenoviral vectors, wherein the viral capsid has been coated with polypeptides, which are capable of stimulating a peptide-specific immune response in a subject and uses thereof. Furthermore, the present invention relates to methods of treating diseases, e.g. cancer, by adenoviral vectors which have been coated by polypeptides causing peptide-specific immune response. Also the present invention relates to a method of coating adenoviral vectors by specific peptides as well as to a method of identifying those peptides suitable for coating the capsid of an adenoviral vector.

Description

[0001] field of invention [0002] The present invention relates to adenoviral vectors and their use, wherein the viral capsid has been coated with a polypeptide capable of stimulating a peptide-specific immune response in an individual. Furthermore, the present invention relates to a method of treating diseases such as cancer by means of an adenoviral vector which has been coated with a polypeptide capable of eliciting a peptide-specific immune response. The present invention also relates to methods of coating adenoviral vectors with specific peptides and methods of identifying those peptides suitable for coating adenoviral vector capsids. [0003] Background of the invention [0004] Cancer is a deadly disease that needs more effective treatments. Oncolytic viruses are of interest because of their potential to be safer and more effective than any other standard therapy. However, in cancer patients, the overall therapeutic effect is smaller. There has been a lot of research...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K47/46C12N15/86G01N33/574G01N33/68A61K35/761A61K47/42A61K47/48A61P35/00A61P35/02
CPCA61K35/761A61K39/0011A61K47/42A61K47/46C12N15/86G01N33/574G01N33/68G01N2333/47C12N2710/10043A61K2300/00A61K2039/522A61P31/00A61P31/12A61P35/00A61P35/02A61P37/04A61K2039/6075C12N2710/10042A61K39/235A61K2039/525C12N15/861A61K2039/60
Inventor 文森佐·史汝乐马库斯·瓦哈科斯凯拉马里·黑尔维尼克里斯蒂安·卡帕索
Owner 瓦洛治疗公司
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