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A serum-free cell freezing medium

A cryopreservation liquid and serum-free technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of low cell recovery rate, increased pollution, and complex components, so as to simplify the cryopreservation procedure and improve recovery High efficiency and wide adaptability

Active Publication Date: 2020-09-01
YOCON BIOLOGY TECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] When the cell cryopreservation solution is used to preserve the cells, the cells are protected by the cryoprotectant during the cryopreservation process. The traditional cell protectant is only an intracellular protectant. The cells are easily damaged by ice crystals during cryopreservation, and the recovery rate of the cells is low. inability to effectively protect cells
In addition, the cell cryopreservation solution in the prior art usually contains animal-derived substances with complex components, which has potential risks in clinical application and increases the chance of contamination

Method used

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  • A serum-free cell freezing medium
  • A serum-free cell freezing medium
  • A serum-free cell freezing medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Preparation of cell freezing solution

[0063] It is composed of components with the following concentrations, and the units of each concentration are mg / L unless otherwise specified:

[0064] L-Arginine 200;

[0065] CuSO 4 ·5H 2 O 0.001;

[0066] L-Asparagine 50;

[0067] L-aspartic acid 20;

[0068] L-glutamic acid 20;

[0069] Ni(NO 3 ) 2 ·6H 2 O 0.0001;

[0070] L-Glutamine 250;

[0071] ZnSO 4 ·7H 2 O 0.3;

[0072] Glycine 10;

[0073] CoCl 2 ·6H 2 O 0.004;

[0074] L-histidine 20;

[0075] NaSiO 3 9H 2 O 0.005;

[0076] L-isoleucine 50;

[0077] Na 3 VO 4 12H 2 O 0.002;

[0078] L-lysine hydrochloride 40;

[0079] SnC1 2 2H 2 O 0.00005;

[0080] L-methionine 20;

[0081] Na 2 SeO 3 0.006;

[0082] L-phenylalanine 20;

[0083] FeSO 4 ·7H 2 O 1.0;

[0084] L-proline 20;

[0085] Glucose 2500;

[0086] L-serine 30;

[0087] Vitamin C 0.500;

[0088] L-threonine 20;

[0089] Paraben 1.0;

[0090] L-tryptophan 10; ...

Embodiment 2

[0104] Example 2 Preparation of cell cryopreservation solution

[0105] It is composed of components with the following concentrations, and the units of each concentration are mg / L unless otherwise specified:

[0106] L-Arginine 100;

[0107] CuSO 4 ·5H 2 O 0.0005;

[0108] L-asparagine 75;

[0109] L-aspartic acid 30;

[0110] L-glutamic acid 10;

[0111] Ni(NO 3 ) 2 ·6H 2 O 0.00002;

[0112] L-Glutamine 500;

[0113] ZnSO 4 ·7H 2 O 0.6;

[0114] Glycine 5;

[0115] CoCl 2 ·6H 2 O 0.001;

[0116] L-histidine 30;

[0117] NaSiO 3 9H 2 O 0.01;

[0118] L-isoleucine 25;

[0119] Na 3 VO 4 12H 2 O 0.0005;

[0120] L-lysine hydrochloride 60;

[0121] SnC1 2 2H 2 O 0.0001;

[0122] L-Methionine 10;

[0123] Na 2 SeO 3 0.002;

[0124] L-Phenylalanine 30;

[0125] FeSO 4 ·7H 2 O 0.1.6;

[0126] L-proline 10;

[0127] Glucose 1000;

[0128] L-serine 45;

[0129] Vitamin C 0.704;

[0130] L-threonine 10;

[0131] p-hydroxybenzoic acid 0.5;

...

Embodiment 3

[0145] Example 3 Preparation of cell cryopreservation solution

[0146] It is composed of components with the following concentrations, and the units of each concentration are mg / L unless otherwise specified:

[0147] L-Arginine 300;

[0148] CuSO 4 ·5H 2 O 0.005;

[0149] L-asparagine 25;

[0150] L-Aspartate 10;

[0151] L-glutamic acid 30;

[0152] Ni(NO 3 ) 2 ·6H 2 O 0.0002;

[0153] L-Glutamine 100;

[0154] ZnSO 4 ·7H 2 O 0.06;

[0155] Glycine 15;

[0156] CoCl 2 ·6H 2 O 0.008;

[0157] L-histidine 10;

[0158] NaSiO 3 9H 2 O 0.001;

[0159] L-isoleucine 75;

[0160] Na 3 VO 4 12H 2 O 0.005;

[0161] L-lysine hydrochloride 20;

[0162] SnC1 2 2H 2 O 0.00001;

[0163] L-methionine 30;

[0164] Na 2 SeO 3 0.01;

[0165] L-Phenylalanine 10;

[0166] FeSO 4 ·7H 2 O 0.2;

[0167] L-proline 30;

[0168] Glucose 4000;

[0169] L-serine 15;

[0170] Vitamin C O.176;

[0171] L-threonine 30;

[0172] p-Hydroxybenzoic acid 1.5;

[0173...

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Abstract

The invention discloses a serum-free cell cryopreservation solution. The serum-free cell cryopreservation solution is prepared from amino acid, inorganic salt, albumin, extracellular cryoprotectant and the like. The serum-free cell cryopreservation solution contains double protective components and can synchronously protect cells from being damaged by ice crystals from cell interiors to cell exteriors during cryopreservation, the recovery rate of the cells is greatly improved, and the serum-free cell cryopreservation solution is suitable for preservation of wide cell lines, wherein the serum-free cell cryopreservation solution can be used in long-term cell preservation, cell bank establishment, scientific-research cell strain preservation and long-term preservation of clinical mesenchymal stem cells, mononuclear cells, immune cells and all related mammal cells; meanwhile, the serum-free cell cryopreservation solution is a cryopreservation solution completely without serum, the situation is avoided that heterogeneous and exogenous components are introduced in clinical application, and the serum-free cell cryopreservation solution is safer.

Description

technical field [0001] The invention relates to a serum-free cell cryopreservation solution. Background technique [0002] When the cell cryopreservation solution is used to preserve the cells, the cells are protected by the cryoprotectant during the cryopreservation process. The traditional cell protectant is only an intracellular protectant. The cells are easily damaged by ice crystals during cryopreservation, and the recovery rate of the cells is low. Cannot effectively protect cells. In addition, the cell cryopreservation solution in the prior art usually contains animal-derived substances with complex components, which has potential risks in clinical application and increases the chance of contamination. Contents of the invention [0003] Aiming at the above-mentioned prior art, the present invention provides a serum-free cell cryopreservation solution, which can be used for long-term preservation of cells, can be applied to the establishment of cell banks, preservat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 曲宝赤
Owner YOCON BIOLOGY TECH CO
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