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Immune cell cryopreservation liquid and immune cell cryopreservation method

A technology of immune cells and cryopreservation solution, applied in the field of cell biology, which can solve the problems of immune cells that cannot be stored and transported for a long time, and achieve the effect of ensuring physiological functions and biological characteristics, a large number of cell proliferation, and high safety

Inactive Publication Date: 2017-08-18
DONGGUAN BOALAI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a cryopreservation method for immune cells, which can effectively solve the problems of immune cells that cannot be stored and transported for a long time, and realize the application of local collection, remote preparation and instant recovery of immune cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] An immune cell cryopreservation solution, the immune cell cryopreservation solution includes the following components:

[0053] Dimethyl sulfoxide 4mL

[0054] Dextran 2mL

[0055] Cell culture medium 10mL

[0056] Human serum albumin 1mL

[0057] Human autologous plasma 60mL.

[0058] The cell culture medium is X-VIVO15 medium.

[0059] The preparation method of the human autologous plasma is as follows: put human peripheral blood in a sterile centrifuge tube, centrifuge at 1500rpm for 8min, take the upper plasma and inactivate it at 56°C for 32min, then transfer it to a new centrifuge tube at 2500rpm Centrifuge at a rotating speed for 15 minutes, and absorb the supernatant, which is the human autologous plasma for cryopreservation.

[0060] The immune cell cryopreservation solution also includes 1 g of sugar, which is a mixture of trehalose, α-1,4-glucan, lentinan and glucose in a weight ratio of 1:0.8:1.5:2.

[0061] The immune cell cryopreservation solution al...

Embodiment 2

[0068] An immune cell cryopreservation solution, the immune cell cryopreservation solution includes the following components:

[0069] Dimethyl sulfoxide 5mL

[0070] Dextran 3mL

[0071] Cell culture medium 15mL

[0072] Human serum albumin 2mL

[0073] Human autologous plasma 70mL.

[0074] The cell culture medium is AIM-V medium.

[0075] The preparation method of the human autologous plasma is as follows: human peripheral blood is placed in a sterile centrifuge tube, centrifuged at 1600rpm for 7min, the upper layer of plasma is inactivated at 57°C for 31min, and then transferred to a new centrifuge tube at 2800rpm Centrifuge at a rotational speed for 22 minutes, and absorb the supernatant, which is the human autologous plasma for cryopreservation.

[0076] The immune cell cryopreservation solution also includes 1.5g of sugar, which is a mixture of trehalose, α-1,4-glucan, lentinan and glucose in a weight ratio of 1:0.9:1.8:2.5 .

[0077] The immune cell cryopreserva...

Embodiment 3

[0084] An immune cell cryopreservation solution, the immune cell cryopreservation solution includes the following components:

[0085] Dimethyl sulfoxide 6mL

[0086] Dextran 4mL

[0087] Cell culture medium 20mL

[0088] Human Serum Albumin 3mL

[0089] Human autologous plasma 80mL.

[0090] The cell culture medium is DMEM / F12 medium.

[0091] The preparation method of the human autologous plasma is as follows: human peripheral blood is placed in a sterile centrifuge tube, centrifuged at 1800rpm for 6min, the upper layer of plasma is inactivated at 58°C for 30min, and then transferred to a new centrifuge tube at 3000rpm Centrifuge at a rotating speed for 20 minutes, and absorb the supernatant, which is the human autologous plasma for cryopreservation.

[0092] The immune cell cryopreservation liquid also includes 2 g of sugar, which is a mixture of trehalose, α-1,4-glucan, lentinan and glucose in a weight ratio of 1:1:2:3.

[0093] The immune cell cryopreservation solut...

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Abstract

The invention relates to the technical field of cytobiology, in particular to an immune cell cryopreservation liquid and an immune cell cryopreservation method. The immune cell cryopreservation liquid comprises components as follows: 4-8 mL of dimethyl sulfoxide, 2-6 mL of dextran, 10-30 mL of a cell culture medium, 1-5 mL of human serum albumin and 60-100 mL of autologous plasm. The immune cell cryopreservation liquid can effectively protect immune cells from cryodamage, is high in safety, causes little damage to the cells and can increase recovery and survival rates of the immune cells, the survival rate after recovery can reach 96% or higher, besides, after recovery, cell viability is high, the multiplication number of the cells is large, the cells are not prone to aging, physiological functions and biological characteristics of the immune cells after recovery are guaranteed, microbiological detection indexes meet requirements, survival time of the immune cells is prolonged, and loss of surface antigens of the immune cells is reduced.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to an immune cell cryopreservation solution and an immune cell cryopreservation method. Background technique [0002] Cellular immunotherapy is an emerging and brand-new anti-tumor treatment method with significant curative effect. It makes up for the disadvantages of traditional surgery, radiotherapy and chemotherapy. It has been recognized as the most active and effective tumor comprehensive treatment model in the 21st century. It is a promising treatment method, and it is also the only treatment method in the world that has the hope of completely eradicating tumor cells. [0003] Immune cells refer to cells involved in or related to immune responses, including lymphocytes, dendritic cells, monocytes / macrophages, granulocytes, mast cells, etc. Immune cells can be divided into many types, and various immune cells play important roles in the human body. Mononuclear cells isol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 罗天恩
Owner DONGGUAN BOALAI BIOLOGICAL TECH CO LTD
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