A kind of fertilizer-making and rot-promoting composite bacterial agent and its preparation method and application
A compound inoculant and rot-inducing technology, applied in the preparation of organic fertilizers, biochemical equipment and methods, applications, etc., can solve the problems of deepening soil acidification and hardening, pesticide chemical residues, rotten roots and clumps, etc., and improve phosphorus and potassium fertilizers. The effect of dividing and relieving the hardening problem
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[0059] Its preparation method comprises the following steps:
[0060] (1) Cultivate and ripen soybean rhizobium, Pseudomonas fluorescens, Bacillus cereus, Bacillus colioids, Bacillus subtilis, Paecilomyces lilacinus, and Trichoderma harzianum strains respectively into strain liquids;
[0061] The specific method is:
[0062] Soybean Rhizobium, Pseudomonas fluorescens, Bacillus cereus, Bacillus colioids and Bacillus subtilis were respectively cultured in medium 1, Paecilomyces lilacinus was cultured in medium 2, Trichoderma harzianum was cultured in medium 3 Cultivation, the culture conditions of each strain are: at a temperature of 28-30°C, maintain agitation and oxygenation, cultivate for 48-72 hours, then put all the strains into the seed tank, and the medium in the seed tank is the same as the previous one. The medium in the steps is the same, the weight ratio of the strain to the culture medium in the seed tank is 1:80, maintain the temperature at 28-30°C, stir and oxygen...
Embodiment 1
[0073] A kind of fertilizer-making and rot-promoting composite bacteria agent, including the bacterial classification of percentage by weight and viable bacteria number as shown in table 2:
[0074] Table 2
[0075]
[0076]
[0077] The preparation method of the fertilizer-making and rot-promoting compound bacterial agent is as follows:
[0078] (1) Cultivate and ripen soybean rhizobium, Pseudomonas fluorescens, Bacillus cereus, Bacillus colioids, Bacillus subtilis, Paecilomyces lilacinus, and Trichoderma harzianum strains respectively into strain liquids;
[0079] The specific method is:
[0080] Soybean Rhizobium, Pseudomonas fluorescens, Bacillus cereus, Bacillus colioids and Bacillus subtilis were respectively cultured in medium 1, Paecilomyces lilacinus was cultured in medium 2, Trichoderma harzianum was cultured in medium 3 Cultivation, the culture conditions of each strain are: at 28°C, maintain agitation and oxygenation, after 72 hours of cultivation, put all ...
Embodiment 2
[0101] A kind of fertilizer-making and rot-promoting composite bacteria agent, including the bacterial classification of percentage by weight and viable bacteria number as shown in table 4:
[0102] Table 4
[0103] bacteria Viable count weight percentage soybean rhizobia 4×10 8 cfu / g
15% Pseudomonas fluorescens 4×10 8 cfu / g
15% Bacillus cereus 4×10 8 cfu / g
15% Bacillus glialis 4×10 8 cfu / g
14% Bacillus subtilis 4×10 8 cfu / g
12% Paecilomyces lilacinus 4×10 7 cfu / g
14% Trichoderma harzianum 4×10 7 cfu / g
15%
[0104] The preparation method of the fertilizer-making and rot-promoting compound bacterial agent is as follows:
[0105] (1) Cultivate and ripen soybean rhizobium, Pseudomonas fluorescens, Bacillus cereus, Bacillus colioids, Bacillus subtilis, Paecilomyces lilacinus, and Trichoderma harzianum strains respectively into strain liquids;
[0106] The specific method is:
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