Preservative based on gamma-aminobutyric acid combined with lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and aminobutyric acid, applied in the application field of Agaricus bisporus fresh-keeping, can solve the problems of lack of versatility and reference, and have not seen common use, etc., to achieve good practical value and commercial promotion Application prospect, safe and non-toxic fresh-keeping effect, simple preparation method

Active Publication Date: 2017-08-15
芽米科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] From the above-mentioned application of γ-aminobutyric acid and Lactobacillus plantarum, there are certain differences in the amount of γ-aminobutyric acid and Lactobacillus plantarum depending on their uses and formulations, lacking certain versatility and reference, and In the prior art, there is no relevant report that the two are used together

Method used

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  • Preservative based on gamma-aminobutyric acid combined with lactobacillus plantarum and application thereof
  • Preservative based on gamma-aminobutyric acid combined with lactobacillus plantarum and application thereof
  • Preservative based on gamma-aminobutyric acid combined with lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The antistaling agent based on γ-aminobutyric acid combined with Lactobacillus plantarum provided in this example is prepared by the following steps:

[0038] (1) Culture of Lactobacillus plantarum

[0039] Inoculate the obtained original slant-preserved Lactobacillus plantarum strain into the sterilized MRS broth culture solution, culture at 37°C and 150rpm for 16-24 hours, and then repeat the subculture twice under the same conditions;

[0040] The MRS broth culture solution consists of: peptone 10g / L, beef powder 5g / L, yeast powder 4g / L, glucose 20g / L, sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L, triammonium citrate 2g / L, Tween 801mL / L, and the balance is water;

[0041] (2) Preparation of clear water suspension of Lactobacillus plantarum

[0042] Centrifuge the culture solution of Lactobacillus plantarum in step (1) at 12000rpm for 10min, collect the precipitated bacteria, wash with clean water no ...

Embodiment 2

[0068] The antistaling agent provided by the present embodiment, its preparation method is the same as embodiment 1, only adjusting the number of Lactobacillus plantarum cells in the antistaling agent is 10 7 cfu / mL, the final concentration of GABA in the preservative is 100 μg / mL.

[0069] When utilizing the antistaling agent to process Agaricus bisporus, the processing method is the same as in Example 1, only adjusting the soaking time to be 3min.

[0070] The experimental results are shown in Tables 4 to 6 below.

[0071] Table 4, the influence of different fresh-keeping treatments on the hardness of Agaricus bisporus

[0072]

[0073] Table 5, the effect of different preservation treatments on the L* value of Agaricus bisporus

[0074]

[0075] Table 6, the effect of different fresh-keeping treatments on the weight loss rate of Agaricus bisporus

[0076]

[0077] From the analysis of the data in the above table, it can be seen that with the prolongation of stor...

Embodiment 3

[0079] The antistaling agent provided by the present embodiment, its preparation method is the same as that of embodiment 1, only adjusting the number of Lactobacillus plantarum cells in the antistaling agent is 5 * 10 6 cfu / mL, the final concentration of GABA in the preservative is 150 μg / mL.

[0080] When utilizing the antistaling agent to process Agaricus bisporus, the processing method is the same as in Example 1, only adjusting the soaking time to be 5min.

[0081] The experimental results are shown in Tables 7 to 9 below.

[0082] Table 7, the effect of different fresh-keeping treatments on the hardness of Agaricus bisporus

[0083]

[0084] Table 8, the effect of different preservation treatments on the L* value of Agaricus bisporus

[0085]

[0086] Table 9, the effect of different fresh-keeping treatments on the weight loss rate of Agaricus bisporus

[0087]

[0088] From the analysis of the data in the above table, it can be seen that with the prolongation ...

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Abstract

The invention belongs to the technical field of preservation and particularly relates to a preservative based on gamma-aminobutyric acid combined with lactobacillus plantarum and application thereof in agaricus bisporus preservation. The preservative is lactobacillus plantarum clean water suspension wherein aminobutyric acid is dissolved, cell amount of the lactobacillus plantarum in the suspension is 10<6> to 10<7>cfu/mL, and concentration of the gamma-aminobutyric acid is 100 to 200mug/mL. The preservative disclosed by the invention can effectively inhibit brown strain of sporocarp, can keep hardness of sporocarp and prolongs a storage period of agaricus bisporus. The preservative disclosed by the invention has the advantages of safety, no toxicity and an excellent preservation effect, not only can avoid potential hazards brought by using chemical preservative to human and environment, but also can enhance a nutritional health-care effect of the agaricus bisporus. A preparation method of the preservative disclosed by the invention is simple, is simple to operate and convenient to use, can better conform to current preservative designing and developing directions and has better practical value and popularization and application prospect.

Description

technical field [0001] The invention belongs to the technical field of preservation, and in particular relates to a preservation agent based on gamma-aminobutyric acid combined with lactobacillus plantarum and its application in preservation of Agaricus bisporus. Background technique [0002] Agaricus bisporus not only has high nutritional value, is rich in protein, amino acids, vitamins and minerals, but also has good health benefits such as lowering blood pressure, lowering blood fat, anti-tumor, anti-bacterial, anti-inflammatory and liver protection. According to statistics from the China Edible Fungi Association, the output of Agaricus bisporus in my country reached 2.505 million tons in 2014, accounting for more than 50% of the global output and export volume. However, Agaricus bisporus is very prone to browning after harvesting and causes rapid quality decline. The loss caused by browning accounts for about 15% of the annual output every year. The problem of freshness ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154A23B7/155
CPCA23B7/154A23B7/155A23V2002/00A23V2400/169A23V2200/10A23V2250/038
Inventor 李静李霞董新红李培骏王安建李顺峰高帅平
Owner 芽米科技(广州)有限公司
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