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Tissue culture method for increasing banana survival rate

A technology of tissue culture and survival rate, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as poor fruit, inconsistent harvest, sterility, etc.

Inactive Publication Date: 2017-08-11
北海康维生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most cultivated edible bananas are parthenotriploid AAA, with a high degree of sterility, and mainly rely on asexual reproduction. In production, sucking buds are usually used for reproduction. When the seedlings are insufficient, the underground stems of bananas can also be cut into pieces. Propagate seedlings, but long-term use of this method will accumulate many viruses, such as: bunchy top disease, mosaic disease, etc.
cause serious harm
In addition, poor banana seedlings can lead to problems such as poor growth, poor fruit, and inconsistent harvests

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A kind of tissue culture method improving banana survival rate of the present invention, the steps are as follows:

[0018] (1) Induction culture: select banana suction buds as explants, sterilize the explants, and transfer them to induction medium for cultivation. The formula of the induction medium is: MS medium + 6-BA 12mg / L+pear juice 1.1g / L+NAA 0.55g / L+sucrose 18g / L+agar 6g / L; induction culture conditions: dark culture, temperature 29°C;

[0019] (2) Subculture: transfer the clustered buds after induction into the subculture medium for cultivation; the formula of the subculture medium is: MS medium + 0.06g / L+naphthaleneacetic acid 0.1g / L+apple juice 0.02g / L+sucrose 18g / L+agar 6g / L; the subculture conditions are: sunlight for 16 hours a day, light intensity 900Lux, temperature 29°C;

[0020] (3) Rooting culture: transfer the subcultured banana seedlings to the rooting medium for cultivation; the formula of the rooting medium is: MS medium + 6-BA 0.55g / L + sucrose...

Embodiment 2

[0023] A kind of tissue culture method improving banana survival rate of the present invention, the steps are as follows:

[0024] (1) Induction culture: select banana suckers as explants, sterilize the explants, and transfer them to induction medium for cultivation. The formula of the induction medium is: MS medium + 6-BA 10mg / L+pear juice 1.2g / L+NAA 0.6g / L+sucrose 15g / L+agar 6g / L; induction culture conditions: dark culture, temperature 30°C;

[0025] (2) Subculture: transfer the clustered shoots after induction into the subculture medium for cultivation; the formula of the subculture medium is: MS medium + 2,4-dichlorophenoxyacetic acid 0.08g / L+naphthaleneacetic acid 0.08g / L+apple juice 0.03g / L+sucrose 20g / L+agar 6g / L; the subculture conditions are: sunlight for 15 hours a day, light intensity 1000Lux, temperature 30°C;

[0026] (3) Rooting culture: transfer the subcultured banana seedlings to the rooting medium for cultivation; the formula of the rooting medium is: MS me...

Embodiment 3

[0029] A kind of tissue culture method improving banana survival rate of the present invention, the steps are as follows:

[0030] (1) Induction culture: select banana suckers as explants, sterilize the explants, and transfer them to induction medium for cultivation. The formula of the induction medium is: MS medium + 6-BA 15mg / L+pear juice 1.0g / L+NAA 0.5g / L+sucrose 20g / L+agar 6g / L; induction culture conditions: dark culture, temperature 28°C;

[0031] (2) Subculture: transfer the clustered shoots after induction into the subculture medium for cultivation; the formula of the subculture medium is: MS medium + 0.05g / L+naphthaleneacetic acid 0.12g / L+apple juice 0.01g / L+sucrose 15g / L+agar 6g / L; the subculture conditions are: sunlight for 18 hours a day, light intensity 800Lux, temperature 28°C;

[0032] (3) Rooting culture: transfer the subcultured banana seedlings to the rooting medium for cultivation; the formula of the rooting medium is: MS medium + 6-BA 0.5g / L + sucrose 20g...

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Abstract

The invention provides a tissue culture method for improving the survival rate of bananas. The steps are as follows: (1) induction culture; (2) subculture; (3) rooting culture; (4) seedling hardening. And provided three culture media and culture conditions used. Applying the method of the invention to carry out tissue culture on bananas, the survival rate of bananas is as high as over 91%.

Description

technical field [0001] The invention relates to a tissue culture method for improving the survival rate of bananas. Background technique [0002] Banana (Musa balbisiana Colla) is one of the important tropical fruits in southern my country, and it is also an important source of economic income in many areas, and many farmers get rid of poverty by growing bananas. Most cultivated edible bananas are parthenotriploid AAA, which have a high degree of sterility and mainly rely on asexual reproduction. In production, sucking buds are usually used for reproduction. When the seedlings are insufficient, the underground stems of bananas can also be cut into pieces. Propagate seedlings, but long-term use of this method will accumulate many viruses, such as: bunchy top disease, mosaic disease, etc. cause serious harm. In addition, poor banana seedlings can lead to poor growth, poor fruit, and inconsistent harvests. Banana tissue culture technology can quickly reproduce a large number...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 官太平
Owner 北海康维生态农业科技有限公司
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