Complement c1inh gene, vector, recombinant strain and protein of grouper oblique and its application

A grouper and protein technology, which is applied in the field of genetic engineering, can solve the problems of pollution, economic blow of artificial breeding of grouper, high morbidity and mortality rate, etc., so as to reduce production cost and improve immunity of grouper. the effect of enriching the gene pool

Active Publication Date: 2020-07-03
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the current rapid climate change disasters and seriously polluted water quality problems, resulting in high morbidity and mortality, it is also a difficult task to deal a serious economic blow to the artificial breeding of grouper solved problem

Method used

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  • Complement c1inh gene, vector, recombinant strain and protein of grouper oblique and its application
  • Complement c1inh gene, vector, recombinant strain and protein of grouper oblique and its application
  • Complement c1inh gene, vector, recombinant strain and protein of grouper oblique and its application

Examples

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Embodiment Construction

[0037]The present invention will be further described below in conjunction with the examples, but not limited thereto. The molecular biology experimental techniques used in the following examples include PCR amplification, plasmid extraction, plasmid transformation, DNA fragment ligation, enzyme digestion, gel electrophoresis, etc., unless otherwise specified, generally operate according to conventional methods. For details, please refer to " Molecular Cloning Experiment Guide (Third Edition) (translated by Sambrook J, Russell DW, Janssen K, Argentine J. Huang Peitang, etc., 2002, Beijing: Science Press), or according to the conditions suggested by the manufacturer.

[0038] 1. Synthesis of the middle fragment of the complement C1INH gene of the oblique-banded grouper

[0039] Analyze a highly homologous fragment of the sequence of the human C1INH gene by sequence analysis software and design a pair of amalgamative primers C1INH-F and C1INH-R, and the upstream primer C1INH-F i...

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Abstract

The invention discloses epinephelus coioides rC1INH-Nonserpin protein. The amino acid sequence of the protein is shown as SEQ ID NO:4, or the amino acid sequence shown as SEQ ID NO:4 is subjected to substitution, deletion and / or one or several amino acid addition and / or tail end modification to have identical or higher-activity protein. The epinephelus coioides complement C1INH gene sequence and functional fragment rC1INH-Nonserpin nucleotide sequences on the C1INH sequence are obtained for the first time; the gene pool of the epinephelus coioides is enriched; the protein can be used for preparing recombinant protein, fish immune preparations or feed additives; the novel theoretical and practice basis is provided for the physiological immunoassay of the epinephelus coioides.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a rC1INH-Nonserpin protein, a gene encoding the protein, a carrier containing the gene and applications thereof; also relates to a complement C1INH protein, a gene encoding the protein, and a gene containing the gene Carriers and their applications. Background technique [0002] Complement plays a regulatory center in the animal immune system regulated by macrophage activating factor, which can effectively improve the immune ability of the body by improving the phagocytosis of immune cells against invading pathogens and simultaneously stimulating the production of superoxidase. However, excessive activation of complement can cause damage to the body. In the complement system, complement C1 inhibitor (C1INH) is the only negative regulatory element among the complement elements. Complement C1INH is a single-chain molecule, generally composed of 478 amino ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/46C12N15/12A61K38/17A61P37/04A23K20/147
CPCA61K38/00C07K14/461
Inventor 王维娜罗盛伟谢仁崇刘媛康欢
Owner SOUTH CHINA NORMAL UNIVERSITY
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