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Separated endogenous antimicrobial polypeptides and application thereof

An antibacterial polypeptide and endogenous technology, applied in the field of bioengineering, can solve the problems of difficult treatment of infectious diseases and reduced effectiveness of antibiotics, and achieve excellent antibacterial effect

Inactive Publication Date: 2017-08-01
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] An increasing number of infectious diseases such as pneumonia, tuberculosis, etc. are becoming more difficult to treat due to the reduction in the effectiveness of antibiotics to treat infections

Method used

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  • Separated endogenous antimicrobial polypeptides and application thereof
  • Separated endogenous antimicrobial polypeptides and application thereof
  • Separated endogenous antimicrobial polypeptides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Entrust Shanghai Keyept Biotechnology Co., Ltd. to synthesize the following sequence by solid phase method:

[0031] SEQ ID NO.5 VTQPLAPVHNPISV

[0032] SEQ ID NO.6 YPVTQPLAPVHNPIS

[0033] SEQ ID NO.7 VTQPLAPVHNPIS

[0034] SEQ ID NO.8 YPVTQPLAPVHNPISV

[0035] The main biological parameters for obtaining the aforementioned polypeptide sequences through online tools are as follows:

[0036] SEQ ID NO.5

[0037] The isoelectric point (pI) is 6.71, the molecular mass (Mw) is 1471.72Da, and has 14 amino acids. The lower relative molecular mass indicates that the antibacterial polypeptide is not easily hydrolyzed by proteases. The instability coefficient is 53.96, indicating that the antibacterial polypeptide is relatively stable and not easily degraded and destroyed by gastric acid. The fat solubility index and hydrophilicity are 125 and 0.443 respectively, showing hydrophobicity.

[0038] SEQ ID NO.6

[0039] The isoelectric point (pI) is 6.74, the molecular m...

Embodiment 2

[0053] The polypeptides described in Example 1 can be used alone, in addition, these polypeptides can also be used in combination of polypeptide compositions. The polypeptide composition contains at least two polypeptides whose sequences are SEQ ID NO.5 to SEQ ID NO.8, preferably at least two polypeptides whose sequences are SEQ ID NO.5 and SEQ ID NO.6.

Embodiment 3

[0055] This embodiment provides a polypeptide described in the foregoing Example 1, or the polypeptide composition described in Example 2 can be prepared into a pharmaceutically acceptable carrier, such as: capsules, tablets, lozenges, pills, dropping pills, In the form of suppositories, sprays, creams, patches, etc.

[0056] Test example 1 antibacterial test

[0057] Synthesis and dilution of antibacterial peptides

[0058] The antibacterial polypeptides β-Casein 213 and β-Casein 211 polypeptides obtained by solid-phase synthesis in Example 1 of this experiment were taken as examples. Take 1 mg of polypeptide, dilute it with sterile double distilled water to 1 mg / ml and store it at -20°C for later use.

[0059] Strains and Culture

[0060] Escherichia coli (E.coli, ATCC25922), Staphylococcus aureus (S.aureus, ATCC25923) and Yersinia enterocolitica (Y.enterocolitica, ACTT23715) were obtained from the American TypeCulture Collection (ATCC) . Inoculate 30 ul of bacteria...

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Abstract

The invention discloses separated endogenous antimicrobial polypeptides and application thereof. The extracted endogenous antimicrobial polypeptides have remarkable antibacterial effect to yersinia enterocolitis, escherichia coli and staphylococcus aureus. Discovered by electron microscope scanning, the separated endogenous antimicrobial polypeptides inhibit bacteria mainly by destroying the integrity of cell membrane of the bacteria and can be utilized to prepare a drug for resisting the yersinia enterocolitis, the escherichia coli and the staphylococcus aureus.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to an isolated endogenous antibacterial polypeptide and its application. Background technique [0002] Since the first antimicrobial peptides (AMPs) were isolated from Agrobacterium in 1939, more than 2,490 antimicrobial peptides have been discovered in different organisms, and new antimicrobial peptides are still being developed and identified. Antimicrobial peptides are a class of oligopeptides composed of 5 to 100 amino acids, which have broad-spectrum antibacterial activity. Their targets include Gram-positive bacteria, Gram-negative bacteria, fungi, viruses, parasites, and tumor cells. It is rapidly becoming a potential therapeutic drug. [0003] The bactericidal mechanism of antimicrobial peptides includes destroying the integrity of the cell membrane, inhibiting the synthesis of proteins, DNA and RNA, or binding to certain specific targets in the cell to kill bacteria. General...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N15/11A61K38/10A61P31/04
CPCA61K38/00C07K7/08Y02A50/30
Inventor 崔县伟郭锡熔季晨博王兴李沄文娟
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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