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Microbial agent and its application in soil improvement of saline-alkali land

A microbial inoculum and microbial technology, applied in microbial-based methods, microorganisms, applications, etc., can solve problems such as unsatisfactory improvement effect, disrupted soil structure, secondary pollution, etc., to enhance soil microbial activity and increase microbial activity. Diversity, the effect of improving the quality of agricultural products

Active Publication Date: 2020-05-22
北京本农科技发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods have good effects and are not limited by soil conditions, but require a lot of manpower, material and financial resources, and require large investment, slow results, and damage soil structure and soil microorganisms; chemical methods are quick, but often expensive and require Complex equipment conditions, disrupted soil structure and easy to cause secondary pollution, etc., are helpless for large-scale pollution. Therefore, these methods cannot fundamentally solve the problem
As people pay more and more attention to environmental protection, it is urgent to find a new way to treat heavy metal-contaminated soil without destroying the physical and chemical properties of the soil, so bioremediation is the preferred method; Microbial agent products are the main products, the product function is single, and the improvement effect is not ideal

Method used

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  • Microbial agent and its application in soil improvement of saline-alkali land
  • Microbial agent and its application in soil improvement of saline-alkali land
  • Microbial agent and its application in soil improvement of saline-alkali land

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This example provides the screening process of the microorganisms in the microbial inoculum of the present invention.

[0049] a. Use nutrient broth medium to enrich the microorganisms in saline-alkali land, 30°C, 180r / min, shake culture for 2 days. Then perform serial dilution: plate on a selective medium plate containing 10% high-concentration sodium chloride, and spread for 10 -5 , 10 -6 The diluted solution was cultured at 30°C for 2 days, and the salt-tolerant colonies growing on the plate were selected, and then streaked and separated on the selective plate, and purified to obtain strains resistant to high concentrations of sodium chloride. Three Bacillus strains were obtained, namely Bacillus pumilus Bn-19, Bacillus amyloliquefaciens Bn-21 and Bacillus subtilis Bn-3.

[0050] b. To enrich the medium (MgSO 4 ·6H 2 O 0.2g / L, NH 4 Cl 1.0g / L, NaHCO 3 5.0g / L, K 2 HPO 4 0.5g / L, NaCl 2.0g / L, peptone 1.5g / L) for light enrichment and anaerobic culture enrichment,...

Embodiment 2

[0053] This example analyzes whether there is antagonism between the strains screened in Example 1.

[0054] Antagonism between microorganisms generally refers to the phenomenon that the life activities of one microorganism or its metabolites inhibit or interfere with the life activities of another microorganism.

[0055] Antagonism experiments were performed on the screened strains in pairs using the inhibition zone test on agar plates. 5 strains without antagonistic effect were screened out, and these 5 strains could be compounded as soil conditioner microbial strains.

[0056] Table 1 Results of pairwise antagonistic experiments of 5 strains

[0057] strain Bn-2 Bn-19 Bn-21 Bn-3 Bn-23 Bn-2 - - - - - Bn-19 - - - - - Bn-21 - - - - - Bn-3 - - - - - Bn-23 - - - - -

[0058] Note: "-" indicates that there is no antagonistic effect between the strains.

Embodiment 3

[0060] This embodiment provides a method for preparing a liquid microbial agent.

[0061] S31. Fermentation and cultivation of strains: industrially ferment 5 strains of strains according to their respective specific selective media to obtain each fermentation liquid, wherein the bacterial content of Bacillus pumilus Bn-19 fermentation liquid is 2×10 9 CFU / mL, the bacterial content of Bacillus amyloliquefaciens Bn-21 and Bacillus subtilis Bn-3 fermentation broth was 1×10 8 CFU / mL, the content of Rhodopseudomonas palustris Bn-2 bacteria is 5×10 8 CFU / mL, the bacterial content of Lactobacillus plantarum Bn-23 fermentation liquid is 5×10 8 CFU / mL.

[0062] S32. Preparation of bacterial agents: Dilute each fermentation liquid with sterile water, and dilute the three kinds of bacillus until the bacteria content is 1×10 7 CFU / mL, equal volumes were mixed to prepare Bacillus inoculum; the photosynthetic bacteria and Lactobacillus plantarum were diluted to 1×10 7 CFU / mL, prepared ...

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Abstract

The invention relates to the technical field of microbe application, specifically relates to a microbial agent and application thereof to saline-alkali land soil improvement. The microbial agent is prepared by compounding bacillus amyloliquefaciens Bn-21, Bacillus subtilis Bn-3,Bacillus pumilus Bn-19, Rhodopseudomonas palustris Bn-2 and lactobacillus plantarum Bn-23, the compounded multiple microbes has different functions, each microbe carries out different microbe metabolic activity, every two bacterial strains have no antagonism, the function of each microbe is relatively stable, can play a different role in a soil conditioner, increase microbe diversity in soil, enhance activity of the microbe in soil, strengthen the effects of the soil conditioner for improving soil physical and chemical properties and improving the soil fertility.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a microbial bacterial agent and its application in saline-alkali soil improvement. Background technique [0002] Saline-alkali soil is a general term for saline soil, alkaline soil and various salinized and alkalized soils. Saline soil refers to the soil in which the soluble salt content in the soil reaches a certain level and can cause obvious harm to the growth and development of plants; the physical properties of alkaline soil soil deteriorate, the soil is highly discrete, swells when wet, hardens when dry, and has poor permeability. hinder the growth and development of crops. There are many places with serious saline-alkali land in my country, and Xinjiang has the most saline-alkali land, most of which are mainly Glauber's salt, which is relatively easy to control; Baicheng and Songyuan in western Jilin, Daqing and Qiqihar in Heilongjiang, Chifeng and Tongliao ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/07C12R1/125C12R1/25C12R1/01
CPCC09K17/14C09K2109/00C12N1/20
Inventor 韩建均赵川王玉文梁雪杰田文佳
Owner 北京本农科技发展有限公司
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