Method of extracting protein from potato starch processing waste liquid

A technology for processing waste liquid and potato starch, which is applied in the field of protein extraction, can solve the problems of protein loss of activity and protein function discount, and achieve the effects of improved function, convenient operation and convenient extraction

Inactive Publication Date: 2017-07-04
嵊州市派特普科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The protein extracted during the preparation process of the invention has lost its activity, and the function of the protein is greatly reduced

Method used

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Examples

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Effect test

Embodiment 1

[0020] A method for extracting protein from potato starch processing waste liquid, comprising the following extraction steps:

[0021] 1) Centrifuge and filter the potato starch processing waste liquid to obtain a preliminary protein liquid;

[0022] 2) Add ethanol with a saturation of 96% to the protein solution, stir and soak to obtain a precipitate;

[0023] 3) After adding deionized water to the precipitate, use macroporous resin to purify. When purifying, first elute with 94% ethanol, and then elute with sterilized water to obtain the eluent;

[0024] 4) The eluate was dialyzed and dried to obtain protein extract.

[0025] The way of centrifugal filtration in step 1 is: the centrifugal force is 2500g, the pore size of the centrifugal sieve is 55 μm, and the fibers and other solid non-protein substances in the waste liquid are removed by centrifugal filtration, and the centrifugal sieve with a pore size of 55 μm is selected to fully remove impurities. At the same time, t...

Embodiment 2

[0031] A method for extracting protein from potato starch processing waste liquid, comprising the following extraction steps: 1) Centrifugally filter the potato starch processing waste liquid, the centrifugal force is 2500g, the pore size of the centrifugal sieve is 65 μm, and the waste liquid is removed by centrifugal filtration For fibers and other solid non-protein substances in the mixture, a centrifugal sieve with a pore size of 65 μm is used to fully remove impurities and at the same time the protein yield is the highest, and a preliminary protein solution is obtained. The relationship between the pore size of the centrifugal sieve and the protein yield is shown in Table 1. Show:

[0032] Table 1 Relationship between centrifugal sieve aperture and protein yield

[0033] Aperture μm 40 50 60 70 80 Protein content% 20.2 32.5 56.4 59.3 61.2 Protein yield% 86.3 88.9 96.4 97.3 98.4

[0034] 2) Add ethanol with a saturation of 95-98% to t...

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Abstract

The invention provides a method of extracting protein from potato starch processing waste liquid. The method includes the following steps: 1) centrifugally filtering the potato starch processing waste liquid to obtain primary protein liquid; 2) adding ethanol, saturation degree being 95-98%, to the protein liquid, and stirring and soaking the protein liquid to prepare a precipitate; 3) adding deionized water to the precipitate and purifying the precipitate with macroporous resin, wherein the liquid is eluted by 90-94% ethanol and then is eluted by sterilized clear water, thereby preparing an eluent; and 4) dialyzing and drying the eluent to obtain a protein extract. The method is convenient, wherein the activity of the extracted protein is not lost, and functions of proteins are improved. The purity of the extracted proteins is high.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a method for extracting protein from potato starch processing waste liquid. Background technique [0002] With the rapid development of society and the improvement of people's living standards, people's demand for protein intake and variety requirements continue to increase, especially the demand for vegetable protein with health benefits is particularly significant. Potato protein has a variety of balanced amino acid components, especially lysine, which is lacking in grains, is extremely rich, comparable to soybean protein, and has extremely high nutritional value. It can be used in health products, biomedicine and food additives, etc. industry. [0003] In the existing potato protein extraction technology, a method for extracting protein from potato starch processing waste liquid CN102863509A, the invention discloses a method for extracting protein from potato starc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K1/16
CPCC07K1/16C07K1/30C07K1/34C07K1/36
Inventor 施春华张美芬
Owner 嵊州市派特普科技开发有限公司
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