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Rapid propagation method of Miao nationality medicinal material helwingia japonica leaves

A technology of green pod leaves and medicinal materials is applied in the field of rapid propagation of Miao nationality medicinal material green pod leaves. , the effect of fast seedling growth and simple process

Inactive Publication Date: 2017-06-23
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the tissue culture technology of green pod leaves at home and abroad, and there is no application for a patent on tissue culture and rapid propagation of green pod leaves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Collection of explants: Select the segmented stems of green viburnum plants that grow vigorously and have no diseases in the field as explants, and immediately carry out water retention and moisturizing treatment after collection and bring them back to the laboratory in time.

[0021] (2) Adventitious bud induction: when step (1) is collected back to the laboratory, the explants are first cleaned 3 times with 10% washing powder solution, rinsed under tap water for 20 minutes and placed in 75% ethanol in an ultra-clean workbench Disinfect in the solution for 15 seconds, rinse with sterile water twice, blot dry with sterile filter paper, place in 0.1% mercuric chloride solution for 15 minutes, rinse with sterile water for 3 times, and blot dry with sterile filter paper Then cut into 1.0-1.5cm segmented stem segments and inoculate them into the adventitious bud induction medium, and culture them in total darkness at 25°C for 25 days to induce the formation of adventitio...

Embodiment 2

[0027] (1) Collection of explants: Select the segmented stems of green viburnum plants that grow vigorously and have no diseases in the field as explants, and immediately carry out water retention and moisturizing treatment after collection and bring them back to the laboratory in time.

[0028] (2) Adventitious bud induction: when step (1) collects the explant back to the laboratory and cleans it 4 times with 10% washing powder solution, rinse it under tap water for 25 minutes and place it in the ultra-clean workbench in 75% ethanol Disinfect in the solution for 23 seconds, rinse with sterile water twice, blot dry with sterile filter paper, place in 0.1% mercuric chloride solution for 24 minutes, rinse with sterile water for 4 times, blot dry with sterile filter paper Then cut into 1.0-1.5cm segmented stem segments and inoculate them into adventitious bud induction medium, and culture them in total darkness at 27°C for 27 days to induce the formation of adventitious buds, with...

Embodiment 3

[0033](1) Collection of explants: Select the segmented stems of green viburnum plants that grow vigorously and have no diseases in the field as explants, and immediately carry out water retention and moisturizing treatment after collection and bring them back to the laboratory in time.

[0034] (2) Adventitious bud induction: when step (1) collects the explant back to the laboratory and washes it 5 times with 10% washing powder solution, rinse it under tap water for 30 minutes and place it in the ultra-clean workbench in 75% ethanol Disinfect in the solution for 30 seconds, rinse with sterile water for 3 times, blot dry with sterile filter paper, place in 0.1% mercuric chloride solution for 20 minutes, rinse with sterile water for 5 times, then blot dry with sterile filter paper Then cut into 1.0-1.5cm segmented stem segments and inoculate them into the adventitious bud induction medium, and culture them in total darkness at 28°C for 30 days to induce the formation of adventiti...

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Abstract

The invention relates to a plant tissue culture method in the agricultural biotechnology, and particularly relates to a rapid propagation method of a Miao nationality medicinal material helwingia japonica leaves. The rapid propagation method comprises the following steps sequentially carried out: obtaining explants of the helwingia japonica leaves; carrying out adventitious bud induction; carrying out subculture of adventitious buds; carrying out rooting culture of the adventitious buds; transplanting test-tube plantlets. An induction culture medium for the adventitious bud induction comprises an MS culture medium, 0.5 to 1.0mg / L of ZT, 0.1 to 0.3mg / L of NAA, 25 to 30g / L of saccharose, 3.5 to 4.0g / L of agar and 0.5 to 1.0g / L of activated carbon; pH of the induction culture medium is 5.4 to 5.8. A transplantation survival rate of test-tube plantlets of the miao nationality medicinal material helwingia japonica leaves obtained by the method disclosed by the invention reaches 91% or more.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of green pod leaves of Miao medicinal materials. Background technique [0002] Helwingia japonica (Helwingia japonica) is a deciduous shrub of the genus Helwingia japonica in the family Cornaceae. There are 5 species of Chrysanthemum genus Chrysanthemum, Chinensis, Tibet, Zhejiang, and Emei. They are mainly distributed in Nepal, Sikkim, Bhutan, northern India, and northern Myanmar in eastern Asia. , Northern Vietnam, China and Japan. All five species are produced in China, and they are mainly distributed in virgin forests above 1,500 meters above sea level in Shaanxi, Henan, the Yangtze River Basin and South China. Due to the sessile female flowers of green viburnum leaves, the flowers and fruits grow on the main vein in the center of the leaf surface, which is also called the flower on the leaf or the fruit on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 梁钧淞杨业容莫昭展韦敏
Owner YULIN NORMAL UNIVERSITY
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