Primers for identifying pathogenic bacteria of tomato neck-root rot and wilt and kit

Abstract

The invention discloses primers for identifying pathogenic bacteria of tomato neck-root rot and wilt and a kit. The primer sequences for identifying pathogenic bacteria of tomato neck-root rot are shown as SEQ ID NO: 1 and SEQ ID NO: 2, and the primer sequences for identifying pathogenic bacteria of tomato wilt are shown as SEQ ID NO: 3 and SEQ ID NO: 4. The primers are strong in specificity and high in detection accuracy, can be used for detecting No. 1 physiological races of fusarium oxysporum tomato neck-root rot specialis and fusarium oxysporum tomato wilt specialis, and is significant for early quick detection of tomato neck-root rot and wilt, soil bacteria community distribution as well as prevention and control of diseases.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a primer and a kit for identifying pathogenic bacteria of tomato neck rot and fusarium wilt. Background technique [0002] Tomato (Solanum esculentum L.) is one of the most important vegetables in my country. It is estimated that the national tomato planting area is more than 10 million mu every year, and the output value is more than 40 billion yuan. Tomato planting area continues to expand, and now it can be planted year-round, and the yield is getting higher and higher, the varieties are more and more colorful, and it has a wide range of uses, which is deeply loved by growers and consumers. At present, the factors restricting tomato production are difficult to predict the market conditions, and the most important ones are serious pests and diseases, which make it difficult to improve the yield and quality of tomatoes. Among them, tomato neck rot (Fusarium crown and ...

AI Technical Summary

Problems solved by technology

In recent years, with the expansion of facility tomato planting area and the increase of planting years, soil-borne diseases caused by tomato neck rot and Fusarium wilt have become more and more serious obstacles to continuous cropping, posing a serious threat to the yield and quality of tomatoes. The Bottleneck of Safe Industrial Production of Tomato

[0003] So far, the classification and identification method based on the morphology of Fusarium is difficult to apply to Fusarium, which is highly prone to mutations. The operation is complicated and the identification accuracy is low, which is easy to cause misdiagnosis, delay the timing of prevention and control, and affect the control effect

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