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Culture medium for streptococcus pneumoniae

A Streptococcus pneumoniae and culture medium technology, applied in the field of microorganisms, can solve problems such as slow proliferation of Streptococcus pneumoniae

Inactive Publication Date: 2017-06-20
天津植草园生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the technical defect of prior art, provides a kind of culture medium for Streptococcus pneumoniae, to solve the technical problem that Streptococcus pneumoniae proliferates slower in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A culture medium for Streptococcus pneumoniae, consisting of the following ingredients in parts by weight: 8 parts of starch, 6 parts of sucrose, 2 parts of potato, 1 part of niacin, 1 part of wolfberry leaf, 1 part of amethyst, and 1 part of Ophiopogon japonicus , 1 part of cysteine, 1 part of casein, 1 part of glucose. The medium is a liquid medium with a pH of 7.

Embodiment 2

[0017] A culture medium for Streptococcus pneumoniae, consisting of the following ingredients in parts by weight: 8 parts of starch, 6 parts of sucrose, 2 parts of potato, 1 part of niacin, 1 part of wolfberry leaf, 1 part of amethyst, and 1 part of Ophiopogon japonicus , 1 part of cysteine, 1 part of glucose. The pH of the medium was 6.

Embodiment 3

[0019] A culture medium for Streptococcus pneumoniae, consisting of the following ingredients in parts by weight: 8 parts of starch, 6 parts of sucrose, 2 parts of potato, 1 part of niacin, 1 part of wolfberry leaf, 1 part of amethyst, and 1 part of Ophiopogon japonicus , 1 part casein. The medium is a liquid medium with a pH of 8.

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PUM

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Abstract

The invention provides a culture medium for streptococcus pneumoniae. The culture medium for streptococcus pneumoniae is prepared from, by weight, 8 parts of starch, 6 parts of saccharose, 2 parts of potato, 1 part of nicotinic acid, 1 part of lycium barbarum leaves, 1 part of amethyst and 1 part of radix ophiopogonis. On this basis, a specific amount of cysteine, casein or glucose can also be added. By adding certain traditional Chinese medicinal materials based on common materials of culture media, a remarkable culture effect is realized unexpectedly. The culture medium is especially suitable for streptococcus pneumoniae culture and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for Streptococcus pneumoniae. Background technique [0002] Streptococcus pneumoniae was first isolated from patient sputum in France and the United States by Louis Pasteur and G.M.Sternberg in 1881, respectively. It is Gram-positive, and the bacteria are like spearheads, diplococci arranged in pairs or in short chains, and some virulent strains have a capsule with a chemical composition of polysaccharides. 5% to 10% of normal people carry this bacterium in the upper respiratory tract, and the virulent strain is an important pathogenic bacterium that causes human diseases. The bacterial strain has certain applications in the field of biotechnology, but the growth rate of Streptococcus pneumoniae is relatively slow in the commonly used culture medium, and at the same time, it is prone to bacterial contamination. Contents of the invention [0003] The pu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/46
CPCC12N1/20
Inventor 刘鼎阔张俊霞张凤洪王立红
Owner 天津植草园生物科技有限公司
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