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Plant cultivating method

A cultivation method and plant technology, applied in the field of plant cultivation, can solve the problems such as the inability of rare and precious plants to adapt to the environment, agricultural production losses, and freezing damage to seedlings, and achieve stress resistance such as saving light costs, increasing the growth rate of seedlings, and resisting diseases. Trait-promoting effects

Inactive Publication Date: 2017-06-20
JIANGMEN HONGHAO SHIYOU BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, for rare and precious plants, in the natural environment, due to the influence of the climate environment, the rare and precious plants cannot adapt to the environment, their survival rate is low, and they are even on the verge of extinction.
When the soil nutrients are unbalanced, it is also prone to deficiency and thin seedlings. Due to the unstable temperature during the seedling cultivation period, the seedlings suffer from freezing damage, which often causes serious losses to agricultural production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A plant cultivation method, comprising the steps of:

[0022] S1. Sterilization method of explants: first rinse the capsules with running water for 20 minutes, scrub the surface of the capsules with washing powder water, rinse 3 times with distilled water, disinfect with 75% alcohol for 3 minutes on the ultra-clean workbench, and then use 8% sodium hypochlorite solution Soak for 6 minutes, shake constantly during the period to make it thoroughly sterilized, rinse with sterile water 3 times, and dry the surface moisture with sterile filter paper for later use;

[0023] S2. Cut open the capsule under aseptic conditions, grab the capsule shell with tweezers, and shake the seeds gently onto the surface of the culture medium;

[0024] S3. Aseptic germination of plant seeds: 1 / 2M medium was used as the basic medium, and 30 L of sucrose and 6.5 g / L of agar powder were added to the medium; different G. The inoculum amount is 0.3g dry weight per seed or rooted seedling;

[002...

Embodiment 2

[0030] A plant cultivation method, comprising the steps of:

[0031] S1. Sterilization method of explants: first rinse the capsules with running water for 30 minutes, scrub the surface of the capsules with washing powder water, rinse 4 times with distilled water, disinfect with 75% alcohol for 4 minutes on the ultra-clean workbench, and then use 8% sodium hypochlorite solution Soak for 8 minutes, shake continuously during the period to make it thoroughly sterilized, rinse with sterile water 5 times, and dry the surface moisture with sterile filter paper for later use;

[0032] S2. Cut open the capsule under aseptic conditions, grab the capsule shell with tweezers, and shake the seeds gently onto the surface of the culture medium;

[0033] S3. Aseptic germination of plant seeds: 1 / 2M medium was used as the basic medium, and 30 L of sucrose and 6.5 g / L of agar powder were added to the medium; different G. The inoculum amount is 0.4g dry weight per seed or rooted seedling;

[0...

Embodiment 3

[0039] A plant cultivation method, comprising the steps of:

[0040] S1. Sterilization method of explants: first rinse the capsules with running water for 35 minutes, scrub the surface of the capsules with washing powder water, rinse 5 times with distilled water, disinfect with 75% alcohol for 5 minutes on the ultra-clean workbench, and then use 8% sodium hypochlorite solution Soak for 10 minutes, shake constantly during the period to make it thoroughly sterilized, rinse with sterile water 6 times, and dry the surface moisture with sterile filter paper for later use;

[0041] S2. Cut open the capsule under aseptic conditions, grab the capsule shell with tweezers, and shake the seeds gently onto the surface of the culture medium;

[0042] S3. Aseptic germination of plant seeds: 1 / 2M medium was used as the basic medium, and 30 L of sucrose and 6.5 g / L of agar powder were added to the medium; different gibberellins were added to the medium before sterilization, and the inoculatio...

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PUM

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Abstract

The invention discloses a plant cultivating method, which mainly comprises the following steps: explant sterilization process, sterile germination of plant seeds, observation of embryo morphology and the process of the embryo breaking through the seed coat, and tissue culture and rapid propagation of the plant. The plant cultivating method reduces the dosage of components of a culture medium, and thus can reduce the production cost; the cultivation time is short with 5-8 days in advance, thereby saving the illumination cost; the cultivation contamination rate is reduced, which is about 3% and conventionally 8% around; and as the culture medium for cultivation is added with organics, the pollution influence of transplantation of seedlings and the culture medium on the environment is reduced.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and more specifically relates to a plant cultivation method. Background technique [0002] At present, for the rare and precious plants, in the natural environment, due to the influence of the climate environment, the rare and precious plants cannot adapt to the environment, their survival rate is low, and they are even on the verge of extinction. When the soil nutrients are unbalanced, it is also prone to deficiency and thin seedlings. Due to the unstable temperature during the seedling cultivation period, the seedlings suffer from freezing damage, which often causes serious losses to agricultural production. Therefore, it is necessary to cultivate rare and precious plants artificially so that they can reproduce rapidly and create value for human beings and the environment. [0003] The concept of plant tissue culture (in a broad sense) is also called in vitro culture, which refers to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N37/06A01P21/00
CPCA01H4/001A01H4/008A01N37/06
Inventor 苏炳豪苏坚宏
Owner JIANGMEN HONGHAO SHIYOU BIOLOGICAL
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