BCR diversity detection kit and application thereof

Abstract

The invention provides a kit for detection BCR diversity. The kit comprises a set of multiple PCR primers. The multiple PCR primer contains upstream primers and downstream primers. The upstream primers are composed of a group of sequences which have a one-to-one correspondence with nucleotide sequences as shown in SEQ ID NO:1- SEQ ID NO: 13. Sequences in the upstream primer have 0-3 more or less nucleotides than the corresponding sequences in the SEQ ID NO:1- SEQ ID NO:13. The downstream primers are composed of a group of sequences which have a one-to-one correspondence with nucleotide sequences as shown in SEQ ID NO:14- SEQ ID NO:17. Sequences in the downstream primer have 0-3 more or less nucleotides than the corresponding sequences in the SEQ ID NO:14- SEQ ID NO:17. By the use of the kit, BCR sequences of human can be efficiently obtained, human specific BCR CDR3 sequences are obtained, and detection rate of low-copy-number B cell clones can be raised.

Description

[0001] related application [0002] This application is a divisional case of Chinese patent application 201510500391.8 with an application date of August 14, 2015, and titled "A Multiplex PCR Primer and Method for Constructing Leukemia Minimal Residual Lesion BCR Library Based on High-throughput Sequencing". technical field [0003] The invention belongs to the field of molecular biology and relates to a gene detection kit, in particular to a BCR diversity detection kit and its application. Background technique [0004] The antigen specificity of T / B lymphocytes is largely determined by the amino acid sequence of the T / B lymphocyte receptor CDR3 region. A large number of V and J gene fragments on the T / B cell locus will produce various rearrangements in the synthesis of receptors. Nucleotides between V-J, V-D and D-J junctions are inserted or deleted independently of the template, and high The frequency variation is similar, which further increases the potential diversity o...

AI Technical Summary

Problems solved by technology

[0006] At present, the acquisition or analysis methods of human BCR genes still need to be improved

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