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Application of sucrose synthase in regulating of development of plant fruits

A technology of sucrose synthase and plants, which is applied in the fields of application, plant products, plant gene improvement, etc., and can solve problems such as unclear function of sucrose synthase

Active Publication Date: 2017-06-13
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the function of sucrose synthase in the process of cucumber fruit development is still unclear

Method used

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  • Application of sucrose synthase in regulating of development of plant fruits
  • Application of sucrose synthase in regulating of development of plant fruits
  • Application of sucrose synthase in regulating of development of plant fruits

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Embodiment 1, the acquisition of sucrose synthase protein and its coding gene

[0101] Sequence analysis, section interception and functional verification were carried out on the genomes of various cucumber varieties, and a sucrose synthase protein was found from Xintaimici cucumber, which was named CsSUS4 protein, as shown in sequence 1 of the sequence list. The gene encoding CsSUS4 protein is named CsSUS4 gene, as shown in sequence 2 of the sequence listing.

Embodiment 2

[0102] Example 2. Analysis of CsSUS4 gene expression and enzyme activity in different parts of cucumber in different periods

[0103] 1. Take the following materials from Xintai Mici cucumber in the following periods:

[0104] Spatial expression sampling: root (R), stem (S), mature leaf (ML), young leaf (YL), male flower (FF) and female flower (MF) on the same cucumber plant.

[0105] Time expression sampling: fruit two days before flowering (-2DAF), fruit on the day of flowering (0DAF), fruit three days after flowering (3DAF), fruit six days after flowering (6DAF) and fruit nine days after flowering (commercial ripe fruit, 9DAF) .

[0106] Three biological replicates were taken for each of the above samples.

[0107] The total RNA of the above materials was extracted, and the first-strand cDNA was synthesized, and the expression of the CsSUS4 gene was detected by qRT-PCR (with the TUA gene as the internal reference gene), and the expression of the CsSUS4 gene was detected b...

Embodiment 3

[0115] Embodiment 3, the acquisition of transgenic plants

[0116] 1. Construction of overexpression vector

[0117] 1. Extract the total RNA of Xintaimi thorn cucumber, and reverse transcribe it into cDNA, use the cDNA as a template, use the primer pair composed of SS4F and SA4R to carry out PCR amplification, and recover the PCR amplification product.

[0118] SS4F: 5'-GC TCTAGA ATGGCTTCTTTGGTGGTAAATCATCATAACGGT-3';

[0119] SA4R: 5'-TCC CCCGGG TTACTTTTTGAATCCGAGATTGGGTGCGCTT-3'.

[0120] In SS4F and SA4R, the underline marks the restriction sites of XbaI and SmaI, respectively.

[0121] 2. Double-digest the PCR amplification product of step 1 with restriction endonucleases XbaI and SmaI, and recover the digested product.

[0122] 3. The vector pBI 121 was double-digested with restriction enzymes XbaI and SmaI, and the vector backbone of about 14750 bp was recovered.

[0123] 4. Ligate the digested product of step 2 with the vector backbone of step 3 to obtain the ov...

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Abstract

The present invention discloses application of sucrose synthetase in regulating of development of plant fruits. The present invention provides a method for cultivation of a transgenic plant, a sucrose synthase encoding gene is introduced into the plant to obtain a transgenic plant, and fruit yield of the transgenic plant is higher than that of a target plant. The sucrose synthase encoding gene may be sucrose synthase gene CsSus4. A key role in the development of cucumber fruits of the sucrose synthase gene CsSus4 is discovered, sink strength can be regulated, and the development of the fruits can be promoted. The good function has guiding significance for effectively improving cucumber yield.

Description

technical field [0001] The invention relates to the application of a sucrose synthase in regulating the development of plant fruit. Background technique [0002] Cucumber is one of the important vegetables widely cultivated in countries all over the world, and it is also one of the five major vegetable crops in my country. Like most higher plants, the photosynthesis of cucumber mainly occurs in green tissues such as mature leaves, fruits and stems, which we call "source". For non-green tissues that do not have photosynthetic ability, such as roots, flowers and seeds, we call them "sinks". Sucrose is the main product of photosynthesis in green plants, and it is also the main transport form of photosynthetic products. Sucrose is transported to the sink organs through the phloem to supply carbon and energy for the growth and development of cells; sucrose metabolism plays an important role in plant development, stress response and yield formation. It plays an important role ma...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/08C12N9/10C12N15/54C12N15/113
Inventor 张振贤范敬伟李翔王虹云眭晓蕾李欣
Owner CHINA AGRI UNIV
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