Rice variety Zhejiang 5 black-streaked dwarf virus resistant site qRBSDV17 and molecular marker method thereof
A technology for rice black-streaked dwarf and black-streaked dwarf disease, applied in the field of agricultural science, can solve the problems of poor repeatability, time-consuming and labor-intensive, and speed up the breeding process of disease-resistant varieties, so as to speed up the breeding process, clear the location, and facilitate identification Effect
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[0031] The methods used in the following examples are conventional methods unless otherwise specified.
[0032] 1. Screening of resistance sources and F2 population construction of Zhejing 5 / Lianjing 3:
[0033] In 2013, a total of 251 micro-core rice varieties from 15 provinces were collected for field induction identification of black-streaked dwarf resistance. The results showed that there were significant differences in the resistance of rice varieties to black-streaked dwarf in different provinces. The japonica rice variety Zhejing No. 5 in Zhejiang has good resistance to black-streaked dwarf disease, and the incidence rate in the field is only 5%, which belongs to the high level of resistance (Southwest Agricultural Journal, 2014, 27 (6): 2365-2369). The rice black-streaked dwarf resistance source Zhejing 5 was used as the female parent, and the rice black-streaked dwarf high-susceptibility variety Lianjing 3 was used as the male parent to prepare a hybrid F1, and a F2 s...
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