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Laboratory bacterium immobilization method

A laboratory-use, bacterial technology, applied in biochemical equipment and methods, methods based on microorganisms, immobilized on/in organic carriers, etc., can solve the problem of low bacterial embedding and difficult long-term operation of continuous reaction systems , poor stability of bacteria immobilized particles, etc., to achieve high survival rate, reduce occupied space, and easy to operate

Inactive Publication Date: 2017-06-13
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims to solve the problems of low bacteria embedding amount, poor stability of bacteria immobilized particles and difficult long-term operation of the continuous reaction system in the existing method, and provides a laboratory-used bacteria immobilization method

Method used

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specific Embodiment approach 1

[0032] Specific implementation mode one: combine figure 1 Illustrate this embodiment, the method for immobilizing bacteria in the laboratory of this embodiment comprises the following steps:

[0033] 1. Carry out bacterial culture, make the bacteria in the logarithmic growth phase, and obtain the bacterial liquid;

[0034] 2. Centrifuge the bacterial liquid at 4°C, 9000-12500rpm to obtain the bacterial cells for immobilization;

[0035] 3. Disperse the thallus obtained in step 2 with physiological saline, then add polyvinyl alcohol solution and mix evenly to obtain a PVA-thallium mixed solution; the amount of thalline in the PVA-thallium mixed solution is 15~20g / L , wherein the amount of thalline is dry weight, and the concentration of polyvinyl alcohol in the PVA-thalline mixed solution is 8~12g / 100mL;

[0036] Four, use bacterium immobilization reaction device to react, add PVA-thalline mixed solution in the glass bottle 7 of bacterium immobilization reaction device, add b...

specific Embodiment approach 2

[0040] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the bacteria described in step one are Clostridium acetobutylicum, Vibrio brasiliensis, Lactobacillus casei, Escherichia coli or Saccharomycescerevisiae . Others are the same as in the first embodiment.

specific Embodiment approach 3

[0041] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: in step 2, the bacteria liquid is centrifuged at 4° C. and 10,000 to 11,000 rpm. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a laboratory bacterium immobilization method. The method solves the problem that the existing method has a low embedding amount of bacteria, causes poor bacterium immobilized particle stability and utilizes a continuous reaction system difficult to be operated for a long time. The method comprises 1, culturing bacteria to obtain a bacterium liquid, 2, centrifuging the bacterium liquid to obtain immobilized bacteria, 3, dispersing the bacteria through physiological saline, adding a polyvinyl alcohol solution into the dispersion liquid and carrying out mixing to obtain a PVA-bacterium mixed solution, 4, carrying out immobilization through a bacterium immobilization reactor to obtain bacterium immobilized particles, and 5, putting the bacterium immobilized particles into ice water and carrying out standing. The method significantly improves the bacterium embedding amount of the immobilized particles to 15 to 20 g / L, can be well molded, is not broken and does not produce bonding. The immobilized particles have good stability. When the bacterium immobilized particles are used in a continuous fermentation system, the system is stable for at least 2 months. The method is used for bacterium immobilization.

Description

technical field [0001] The invention relates to a method for immobilizing bacteria. Background technique [0002] With the rapid development of continuous fermentation research and industrial application, the emergence of immobilization technology can be divided into continuous fermentation period of free cells and continuous fermentation period of immobilized cells. In the continuous fermentation system of free cells, since the free cells are continuously discharged from the system with the fermentation liquid, the concentration of bacteria in the fermenter is low, which makes the fermentation time longer and the production speed slows down. The continuous fermentation using immobilized cells effectively solves the shortcomings of low cell concentration, long fermentation time, and easy washing of cells in the continuous fermentation process of free cells, thereby increasing the cell concentration in the continuous flow system. [0003] Immobilized Microorganism Technology...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/04C12R1/145C12R1/63C12R1/245C12R1/19C12R1/865
CPCC12N11/04C12N11/08
Inventor 黎双飞王月赵敏雅胡章立徐颖陈辉蓉
Owner SHENZHEN UNIV
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