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Destruxin depsipeptide derivative and preparation method and application thereof

A technology for depsipeptides and derivatives is applied in the field of Destruxin-type depsipeptide derivatives and their preparation, which can solve the problems of carcinogenicity, mutagenesis, teratogenicity and the like, and achieves simple production process, short cycle and low product cost. Effect

Active Publication Date: 2017-06-09
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the resistance of plant diseases and insect pests to chemically synthesized pesticides and insecticides is becoming more and more obvious. Mutagenesis) and other outstanding issues

Method used

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  • Destruxin depsipeptide derivative and preparation method and application thereof
  • Destruxin depsipeptide derivative and preparation method and application thereof
  • Destruxin depsipeptide derivative and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Destruxin-like depsipeptide derivatives are shown in formula (1) and formula (2) (Arabic numbers and Greek letters in the structure are carbon atoms).

[0023]

Embodiment 2

[0024] Example 2: Fermentative production and separation and purification of Destruxin-like depsipeptide derivatives:

[0025] 1) Fermentation culture

[0026] Strain culture: According to the conventional culture method of microorganisms, pick a small amount of strain Beauveriafelina AS-70 stored in agar-malt extract medium, inoculate it on the surface of PDA plate, and cultivate it at 28°C for 3 days, as the strain for large-scale fermentation culture, and wait for use .

[0027] Cut off the appropriate amount of bacteria on the surface of the PDA plate, inoculate it into a sterilized Erlenmeyer flask filled with rice culture medium, and let it stand at room temperature for 30 days. Add ethyl acetate to sterilize and set aside.

[0028] The rice culture medium is rice 100g / bottle, peptone 0.6g / bottle, and natural seawater 100mL / bottle.

[0029] 2) Separation and purification of compounds

[0030] The above-mentioned rice culture medium was ultrasonically extracted 3 time...

Embodiment 3

[0039] Embodiment 3: insecticidal activity test

[0040] Traditional screening methods for agricultural insecticidal active substances are mainly based on one or several standard pests (such as armyworm, fruit fly, aphid, corn borer and diamondback moth, etc.) Characteristics, physiological and biochemical responses are used as evaluation indicators to judge the insecticidal activity of the test compound. Although the traditional screening method is close to the results obtained under field conditions, large-scale, purposeful, and step-by-step screening experiments require a large number of target insects as test materials on the one hand, and on the other hand, the cultivation of test insects is not sensitive to temperature and humidity. Environmental factors such as environmental requirements are very strict, thus time-consuming, laborious, costly, time and economy are not strong.

[0041]The use of Artemia as a model organism for the determination of insecticidal activity ...

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Abstract

The invention relates to microbial-source pesticide, in particular to a Destruxin depsipeptide derivative and a preparation method and application thereof. The Destruxin depsipeptide derivative is as shown in formula (I) and formula (II). The Destruxin depsipeptide derivative is prepared by the strain BeauveriafelinaAS-70 through fermentation, culture, extraction and separation. By authentication using technologies such as nuclear magnetic resonance and mass spectrum, the chemical structure of the Destruxin depsipeptide derivative is good in pesticide activity.

Description

technical field [0001] The invention relates to a microbial source insecticide, in particular to a Destruxin-like depsipeptide derivative and its preparation method and application. Background technique [0002] As the disadvantages of large-scale application of traditional chemically synthesized pesticides become increasingly prominent, the public pays more and more attention to food safety and environmental protection. On the one hand, the resistance of plant diseases and insect pests to chemically synthesized pesticides and insecticides is becoming more and more obvious. Mutagenicity) and other outstanding issues. Compared with traditional chemically synthesized pesticides, microbial-derived natural product pesticides have the advantages of high safety for humans, animals, and non-target organisms, good environmental compatibility, and less resistance; moreover, microorganisms are easy to be fermented and cultivated on a large scale, which is convenient for industrial pr...

Claims

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Application Information

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IPC IPC(8): C07K11/02C12P21/04C07K1/22C07K1/20C07K1/16A01N43/90A01P7/00C12R1/645
CPCA01N43/90C07K11/02C12P21/02
Inventor 王斌贵杜丰玉
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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