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Method for rapidly detecting copper ions

A copper ion, rapid technology, applied in the detection field, can solve the problems of loss of activity, high enzyme cost, insufficient detection specificity and accuracy, etc., and achieve the effect of low detection cost, high sensitivity and strong specificity

Inactive Publication Date: 2017-05-31
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme analysis has the characteristics of rapidity and stability, but this method requires special sample pretreatment, and the enzyme is expensive and easily loses its activity due to the influence of the external environment.
Immunoassay is a commonly used detection method, which has the characteristics of rapidity and sensitivity, but heavy metals have small molecular weight and charge tend to have a strong irreversible reaction with biomolecules, and their specificity as an antibody recognition target is not strong; The color method does not require large-scale instruments, is easy to operate, and is suitable for on-site real-time detection, but the specificity and accuracy of the detection are not enough, and most of them are qualitative or semi-quantitative detection

Method used

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  • Method for rapidly detecting copper ions
  • Method for rapidly detecting copper ions
  • Method for rapidly detecting copper ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 0.5μM copper ion solution mixed with 0.1μM Amplex Red fluorescent dye (a); 0.1μM Amplex Red fluorescent dye mixed with 8mM H 2 o 2 After mixing (b); 0.5 μM copper ion solution, 0.1 μM Amplex Red fluorescent dye and 8 mM H 2 o 2 After mixing (c), utilize a fluorescence spectrophotometer to measure its fluorescence intensity (such as figure 1 shown). The excitation wavelength is 540nm and the emission wavelength is 583nm.

Embodiment 2

[0025] After the 0.001-0.5μM copper ion solution is mixed with Amplex Red dye, the fluorescence intensity is measured by a fluorescence spectrophotometer. The excitation wavelength is 540nm and the emission wavelength is 583nm. Taking copper ion concentration as the abscissa, with (F-F 0 ) / (F'-F 0 ) is a fitting curve for the ordinate, where F 0 , F and F' respectively represent the fluorescence intensity of Amplex Red at 584 nanometers without adding copper ions, the fluorescence intensity of Amplex Red after adding different concentrations of copper ions and the fluorescence intensity of Amplex Red at 583 nanometers when adding 0.5 μM copper ions ( Such as figure 2 shown), the linear relationship between the fluorescence response of Amplex Red and the concentration of copper ions in the two areas of 0.001-0.008 μM and 0.008-0.1 μM can be obtained (such as image 3 , Figure 4 shown), according to image 3 Get the linear regression equation of formula (1), according to...

Embodiment 3

[0029] Select Cr 3+ , Fe 2+ , Zn 2+ , Ca 2+ , Mg 2+ , Cd 2+ , Ni 2+ , Pb 2+ , Fe 3+ , Hg 2+ As a competitor, the specificity of the method was investigated. Prepare 0.5 μM Cu 2+ and 0.5 μM other metal ions. respectively with Amplex Red fluorescent dye and 8mM H 2 o 2 Mix and measure the fluorescence value with a fluorescence spectrophotometer. Such as Figure 5 As shown, copper ions can strongly increase the fluorescence of Amplex Red. However, other interfering metal ions have little effect on its fluorescence. It shows that the method is specific to copper ions.

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Abstract

The invention discloses a method for rapidly detecting copper ions mainly based on non-labeled Amplex Red (10-acetyl-3, 7-dihydroxyphenoxazine) fluorescent dye and H2O2. According to the method, after the copper ions, the Amplex Red fluorescent dye and the H2O2 are simultaneously mixed, the fluorescent strength of the dye is enhanced, and the copper ions can be rapidly and quantitatively detected by detecting the fluorescent strength. The method is simple, high in specificity, rapid in detection and capable of meeting on-site rapid and accurate detection requirements.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a rapid detection method for detecting copper ions based on non-labeled Amplex Red dye molecules. Background technique [0002] Copper is one of the essential trace elements for the human body. The lack or excess of copper ion concentration directly affects human health. If the concentration of copper ions is too high, it will affect the normal metabolism of the liver and kidney, causing functional disorders of the gastrointestinal tract; it can be deposited in the brain and heart, causing neurodegenerative diseases; it can be tightly combined with protein active groups, changing the three-dimensional structure of the enzyme or Substitute the required metal cofactors in the enzyme, thereby making it lose its catalytic activity and causing toxicity to organisms and humans. [0003] Copper in organisms and humans can be ingested through food such as water in daily li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/6421
Inventor 刘亚青高金婷王硕林晓东邓健康
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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