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Method for detecting hemoglobin based on GQDs (graphene quantum dots)

A technology of graphene quantum dots and hemoglobin, which is applied in the field of detection of hemoglobin based on graphene quantum dots, can solve the problems of complex operation steps, large operation errors, and long analysis time, and achieve good fluorescence stability, low cost, sensitive and efficient detection Effect

Inactive Publication Date: 2017-05-31
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods for detecting hemoglobin include electrochemical method, absorption spectrometry method, fluorescence analysis method and high performance liquid chromatography, etc., but the existing methods have disadvantages such as complicated operation steps, large operation errors, and long analysis time.

Method used

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  • Method for detecting hemoglobin based on GQDs (graphene quantum dots)
  • Method for detecting hemoglobin based on GQDs (graphene quantum dots)
  • Method for detecting hemoglobin based on GQDs (graphene quantum dots)

Examples

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Embodiment 1

[0029] Example 1: The degree of fluorescence quenching of graphene quantum dots by hemoglobin is related to its concentration. The specific process is as follows:

[0030] 1. Prepare graphene quantum dot solution: Disperse the synthesized graphene quantum dots in a phosphate buffer (concentration of 0.01 mol / L, pH value of 7.4) to obtain a graphene quantum dot solution (concentration of 20 mg / L) );

[0031] 2. Prepare hemoglobin solution: fully dissolve hemoglobin in phosphate buffer (concentration of 0.01mol / L, pH value of 7.4) to obtain a hemoglobin solution (concentration of 0-10μmol / L);

[0032] 3. Prepare standard solution: Take a certain amount of the solution prepared in step 1 and step 2, mix thoroughly and add phosphate buffer (concentration of 0.01 mol / L, pH value of 7.4) to prepare a set of blank standard samples. Standard solutions with different known hemoglobin concentrations; the final concentration of graphene quantum dots is 20mg / L, and the final concentration gradi...

Embodiment 2

[0035] Example 2: Establish the working curve of graphene quantum dots to detect hemoglobin, the specific process is as follows:

[0036] According to the results obtained in Example 1, we selected hemoglobin with a concentration range of 0-0.5 μmol / L for further experiments. Prepare graphene quantum dot solution (concentration is fixed at 20mg / L), the hemoglobin concentration is 0, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5μmol / L, mix the two to make 7 standard solutions, and Detect the fluorescence spectrum of the standard solution with a fluorescence spectrometer under the condition that the emission wavelength is 368nm, take the fluorescence intensity value F at the wavelength of 470 nm, and calculate the corresponding fluorescence quenching value △F=F 0 -F, △F and the concentration of hemoglobin C have a good linear relationship, draw the C-△F curve, and used as the graphene quantum dots to detect hemoglobin working curve. (For specific results, see image 3 ). Such as image 3 As shown...

Embodiment 3

[0037] Example 3: Using the present invention to determine the content of hemoglobin in a simulated actual sample, the specific process is as follows:

[0038] Under the same experimental conditions, based on the common hemoglobin iron supplement health products in the market, a mixed system containing hemoglobin was simulated, and 20.9 mg / L L-arginine, 22.0 mg / L L-lysine, 39.5 Independent fluorescence quenching experiments were performed on mg / L glucose, 45.3 mg / L sucrose, 44.0 mg / L vitamin C, 37.7 mg / L starch, and 5 mg / L hemoglobin to obtain a relative fluorescence intensity map. (For specific results, see Figure 4 ). The experimental results show that only hemoglobin in the above components makes the graphene quantum dots significantly quenched; at the same time, in the mixed system, the overall quenching degree of the system is equivalent to the quenching degree of hemoglobin alone (96.9%), which indicates that the graphene quantum dots Point probe detection of hemoglobin ...

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Abstract

The invention relates to a method for detecting hemoglobin based on GQDs (graphene quantum dots). The method comprises two main points as follows: (1) establishment of a linear relation: the fluorescence intensity of the GQDs is weakened with an increase of the concentration of a hemoglobin solution according to a fluorescence spectrogram, and a fluorescence quenching value and the concentration of the hemoglobin solution have a good linear relation; (2) detection of a simulation sample: the fluorescence intensity of a to-be-detected hemoglobin solution is detected, and the content of hemoglobin in the to-be-detected hemoglobin solution is determined according to the linear relation. Meanwhile, the method can also be applied to content detection of hemoglobin in a mixed system, and a result proves that the method has the advantage of high-sensitivity detection, thereby having a broad application prospect.

Description

Technical field [0001] The invention relates to a method for detecting hemoglobin based on graphene quantum dots. Background technique [0002] Hemoglobin (Hemoglobin) is the most widely distributed protein in the biological world. It acts to transport oxygen and decompose H in various organisms. 2 O 2 , Energy generation and electron transfer. Abnormal hemoglobin content can cause a variety of diseases. Therefore, the determination of blood hemoglobin content is of great significance for clinical diagnosis and physiological research. [0003] In recent years, graphene quantum dots (graphene quantum dots), as a new type of fluorescent probe, have attracted widespread attention in many research fields. Graphene quantum dots have the advantages of good water solubility, good biocompatibility, strong fluorescence stability, and high quantum yield. These superior properties make graphene quantum dot fluorescent probes widely used in biochemical analysis, imaging analysis, and environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 王艳丽邢晓军姚晨婕丁琳黄雅男吴明红
Owner SHANGHAI UNIV
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