Method for constructing Dolmen structure based on DNA origami template and gold nanorods

A technology of nano-gold rods and paper templates, applied in the field of detection and analysis, to achieve the effect of low experimental conditions and low cost

Active Publication Date: 2017-05-31
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there has been no research and patent reports on the use of the Bottom-Up m...

Method used

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  • Method for constructing Dolmen structure based on DNA origami template and gold nanorods
  • Method for constructing Dolmen structure based on DNA origami template and gold nanorods
  • Method for constructing Dolmen structure based on DNA origami template and gold nanorods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Regarding the preparation of nano-gold rods with specific sizes by the seed crystal growth method. Preparation of seed crystals:

[0059] (1) Stir and mix 10mL aqueous solution of cetyltrimethylammonium bromide with a molar concentration of 0.1mol / L and 0.25mL aqueous chloroauric acid solution with a molar concentration of 0.01mol / L, and quickly add 0.6mL of it through an ice-water bath The molar concentration is 0.01mol / L sodium borohydride aqueous solution, vigorously stirred for 2min;

[0060] (2) The mixed solution obtained in step (1) was left to stand at 30° C. for 2 hours to react.

Embodiment 2

[0061] Example 2. Regarding the preparation of specific size nano-gold rod growth solution by seed crystal growth method:

[0062] (1) 40mL molar concentration is 0.1mol / L cetyltrimethylammonium bromide aqueous solution and 2mL molar concentration is 0.01mol / L chloroauric acid aqueous solution mixing, stirs;

[0063] (2) Add 0.3 mL of silver nitrate solution with a molar concentration of 0.01 mol / L and 0.8 mL of hydrochloric acid solution with a molar concentration of 1 mol / L to the solution in step (1) while stirring at 30° C., and stir for 5 min;

[0064] (3) Add dropwise 0.30 mL of ascorbic acid solution with a molar concentration of 0.1 mol / L to the solution in step (2) while stirring at 30° C., the solution changes from yellow to colorless rapidly, and stir for 2 min;

[0065] (4) Add 0.08 mL of 5-fold seed crystal diluent dropwise to the solution in step (3) while stirring at 30° C., stir for 2 min, and let stand to react for 12 h;

[0066] (5) The solution changes from...

Embodiment 3

[0068] Example 3, about the preparation of gold nanorods modified by ssDNA1:

[0069] Take 150 μL of gold nanorod solution in Example 2 to prepare ssDNA1-modified gold nanorods:

[0070] (1) Centrifuge and wash 150 μL nano-gold rod solution twice, centrifuge at 4500 rpm for 10 minutes, and disperse the centrifuged product into 100 μL ultrapure water;

[0071] (2) Take 5 μL of 100 μM ssDNA1 and add it to the solution in step (1), and at the same time add 1.5 μL of 1% sodium dodecyl sulfate solution, oscillate and mix, and place it in a 37°C, 250rpm Constant temperature shaker, shake and incubate for 4 hours;

[0072] (3) Add 2 mol / L sodium chloride solution dropwise to the solution in step (2), add 2.5 μL each time, and add dropwise 4 times at an interval of half an hour. A constant temperature shaker at 250rpm, shaking and incubating for 8h;

[0073] (4) After the reaction in step (3), concentrate the ssDNA1-modified gold nanorods obtained in step (3) by centrifugal purific...

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Abstract

The invention discloses a method for constructing a Dolmen structure based on a DNA origami template and gold nanorods. The method comprises the following steps: firstly, preparing gold nanorods with a specific size by virtue of a seed crystal growth method; secondly, preparing specifically designed rectangular DNA origami; and finally, adding the gold nanorods according to a mole ratio of the gold nanorods to the DNA origami of 5:1, and performing cyclic gradient annealing at 45-20 DEG C to enable the gold nanorods with the specific size to be hybridized with the specifically designed rectangular DNA origami and the Dolmen structure to be constructed with the gold nanorods. The invention also provides a preparation process for preparing the gold nanorods with the specific size by virtue of the seed crystal growth method and preparing the gold nanorods modified by a nucleotide sequence shown by ssDNA1. According to the method provided by the invention, characterization is performed with the help of agarose electrophoresis and a transmission electron microscope, a gold nanorod assembly can be positioned with a scanning electron microscope together easily by use of a characteristic that the gold nanorod assembly has a special color in a dark field image of a dark field microscope, and compared with an etching means, the method provided by the invention has the advantages of being simple, convenient and reliable, having a low cost and relatively low experiment condition requirements, and the like.

Description

technical field [0001] The invention belongs to the field of detection and analysis, and in particular relates to a method for constructing a Dolmen structure based on a DNA origami template nano-gold rod and an application thereof. Background technique [0002] Paul Rothemund, a senior researcher at the California Institute of Technology, invented "DNA origami", an innovative bottom-up assembly technique. First draw the shape of the folded object with the help of nanometer instruments, and then fill the shape with the long folded DNA chain, and the short DNA chain is the "thumbtack" that fixes the long folded DNA chain. Rothemund uses a computer to calculate the number of short DNA strands required for each work, and then "nails" these short DNA strands to the scaffolds formed by the long strands to form various patterns. This technology has the advantages of simple process, easy operation and high yield, and has been widely used in biosensing, material detection, single-m...

Claims

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Application Information

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IPC IPC(8): G01N21/49G01N27/447G01N23/04B22F9/24B22F1/00B82Y30/00B82Y40/00
CPCG01N21/49G01N23/04G01N27/447B82Y30/00B82Y40/00B22F9/24B22F1/07B22F1/054B22F1/142
Inventor 樊春海汪联辉王旭晁洁
Owner NANJING UNIV OF POSTS & TELECOMM
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