A tick nest PCR specific primer and tick nest PCR identification method

Abstract

The invention provides a tick nest type PCR (Polymerase Chain Reaction) specific primer and a tick nest type PCR identification method. A more sensitive nest type PCR method is adopted; a single tick, even a trace genome DNA (Deoxyribonucleic Acid) extracted from partial tissue organs of the tick, can be used as a template and is used for identifying; somatotype and system evolution analysis on the tick is carried out on a gene level; in the nest type PCR identification method, a first round only needs 10 cycles and a second round needs 25 cycles, so that time needed by two turns of simple common PCR is shortened.

Description

technical field [0001] The invention specifically relates to the technical field of biology, in particular to a tick nest PCR specific primer and a tick nest PCR identification method. Background technique [0002] Ticks are obligate, non-permanent medical arthropods that parasitize terrestrial vertebrates and can cause harm to the host by directly biting and releasing toxins or carrying pathogens. In the investigation of tick species at entry and exit ports, the identification and evolutionary typing of ticks are of great significance. At present, the identification of tick species in my country mainly relies on morphological methods, and there is no simple and stable molecular biological method. Contents of the invention [0003] In order to solve the defects and deficiencies of the prior art, the present invention provides a tick-nest-type PCR-specific primer and a tick-nest-type PCR identification method, which can quickly and stably identify tick species at a low DNA...

AI Technical Summary

Problems solved by technology

At present, the identification of tick species in my country mainly relies on morphological methods, and there is no simple and stable molecular biological method.

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