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Standby form of colletotrichum acutatum laboratory material, and preparation and application method thereof

A kind of anthracnose bacteria and laboratory technology, applied in the field of plant pathology, can solve the problems of delay and inability to carry out in time, and achieve the effect of convenient and fast work and standardization

Inactive Publication Date: 2017-05-31
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This means that the relevant work process needs to be delayed and cannot be carried out in time

Method used

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Examples

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Effect test

Embodiment 1

[0036] Applying the present invention to a laboratory material of anthracnose bacteria in a standing form and its preparation and application method, preparing and cultivating the laboratory standing form material of mango anthracnose bacteria strain Cg-1 with conidia as the thallus; preparation and application of bacterial strain Cg -1 The standing form of materials is operated according to the following steps:

[0037] 1. Preparation and culture of conidia

[0038] Use a triangular flask as a culture device, and put potato glucose medium as a spore-producing medium. The composition ratio of this medium is: 200g of potatoes, 20g of glucose, 20g of agar, and 1000mL of water; The mycelium was transplanted into the spore-forming medium in the Erlenmeyer flask, and after culturing for 5 days at a temperature of 28° C., the colonies on the surface of the medium formed a large number of conidia.

[0039] 2. Preparation of stock form of anthracnose bacteria material

[0040] Injec...

Embodiment 2

[0050] Applying the present invention's standing form of anthracnose bacteria laboratory material and its preparation and application method, preparing and cultivating the laboratory standing form material of mango anthracnose bacteria strain Cg-2 with conidia as thallus; preparation and application of mango The standing form of the anthracnose bacterial strain Cg-2 material is implemented by step 1 to step 5 of embodiment 1, and the difference is that the bacterial strain used in step 1 is Cg-2, and step 2 obtains the standing spores of mango anthracnose bacterial strain Cg-2 liquid.

[0051] Use a pipette to quantitatively absorb 5 μL of the standing spore liquid, transplant it to the middle of the potato glucose culture plate, and cultivate it at 28°C for 2 days, and it can grow into a typical small colony.

[0052] Take 10 μL of the standing spore liquid and transplant it to the potato glucose medium plate, and spread the spore liquid evenly on the plate surface with a ste...

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PUM

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Abstract

The invention discloses a standby form of a colletotrichum acutatum laboratory material, and a preparation and application method thereof. A pure and non-pollution conidium of colletotrichum acutatum is used as the standby form of the laboratory material, and the conidium in the standby form is stored in sterile water. The preparation and application method of the standby form comprises the following steps of (1) using a triangular flask as a culture vessel for preparing the conidium of the colletotrichum acutatum; (2) preparing the standby form of the colletotrichum acutatum material; (3) calibrating the content of the conidium in the standby form of the colletotrichum acutatum material; (4) daily storing the standby form of the colletotrichum acutatum material; (5) applying the standby form of the colletotrichum acutatum material. The standby form of the colletotrichum acutatum laboratory material, and the preparation and application method thereof provided by the invention has the advantages that (1) the growth of the conidium in the standby form can be suspended, but can be back to normal after the conidium is transferred to a culture medium; (2) the conidium in the standby form can be applied in related work at any time, a thallus material can be directly provided for a test link requiring the conidium, and the working procedure of repeatedly preparing and culturing the conidium when a mycelium is used as a standby material can be omitted, so that the work can be carried out conveniently and quickly; (3) the triangular flask is used as the culture vessel, so that the non-pollution conidium liquid can be easily prepared and obtained; (4) the conidium content of the standby conidium liquid can be calibrated, the conidium can be taken and used accurately and quantitatively when being applied, and the standardization of the technical method is facilitated.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a standing form of an anthrax laboratory material and a preparation and application method thereof. Background technique [0002] Anthracnose is an important disease on many crops, often causing great economic losses on many crops. The pathogenic bacteria that cause anthracnose are fungi of the genus Colletotrichum, and the common one is Colletotrichum mgloeosporioides. Anthrax bacteria are easy to grow on ordinary culture medium, and mycelium is developed; in laboratory work, anthrax bacteria are usually cultivated into slant colonies with test tube slant medium, and the mycelium materials of the colonies are used as standing materials for daily work. The starting point of the relevant research work is the mycelium material as the starting point, and through the transplantation of the standing material mycelium, the expanded cultivation or the research work of specifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/645
Inventor 张君成张丽丽蒲相君
Owner GUANGXI UNIV
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