Wireless-programmed illumination system and application for high-throughput optogenetics cell research

Abstract

The invention discloses a wireless program-controlled illumination system used for cell high-throughput research of optogenetics. The system comprises an upper computer and control software, a wireless communication module, a lower computer central processor, a lower computer register chip set, a lower computer light emitting diode group and a constant-voltage direct-current power supply. The device provided by the invention is used for the research for the optogenetics signal channel of the in-vitro large-scale culture of cells, especially the research for drug molecule screening relevant to the signal channel, the problem that in the current relevant researches, the devices for realizing high-throughput, multi-intensity, long-time and multi-factor illumination in-vitro stimulation are not available is solved, and the accuracy and experiment efficiency of the light stimulation system in the optogenetics research are effectively improved. In addition, the cell culture light activating conditions acquired based on the system provided by the invention, such as the illumination intensity and the light activating time, have wide application prospects for the optogenetics research of the cells in the future.

Description

technical field [0001] The invention belongs to the field of integrated electronic circuits and optogenetics, and in particular relates to a wireless program-controlled illumination system and its application for high-throughput research of optogenetics cells. Background technique [0002] Optogenetics technology is a new multidisciplinary biotechnology that has developed rapidly in recent years and integrates optics, genetic engineering and electronic engineering. The use of optogenetic technology to control cell activities has the advantages of non-invasive, non-invasive, high temporal and spatial resolution, quantitative repeatability, and simple use. It has been more and more widely used in neuroscience, cell biology, signaling pathways and other research fields. [0003] Up to now, neurons have been stimulated locally at fixed points with fiber-coupled diodes (such as patent CN201510654472.3) or multi-point light stimulation with micro-LED arrays (such as Kwon K, Li W. ...

AI Technical Summary

Problems solved by technology

[0004] However, in the more emerging field of signaling pathway research, the photostimulation system is still very simple (such as Bugaj L J, Choksi A T, Mesuda C K, et al. Optogenetic protein clustering and signaling activation in mammalian cells[J]. Nature methods, 2013, 10(3 ):249-252.): ①The number of lamp beads is small, and high-throughput molecular screening of signaling pathways cannot be carried out; ②The cells can only be irradiated with a single brightness to stimulate cells, and the brightness cannot be finely adjusted from the software to explore the light intensity and signal pathway activation degree; ③ communicate with computer through physical connection, it is inconvenient to study the long-term irradiation and cultivation of cells in the incubator environment

④At the same time, only one kind of light can be used for stimulation

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