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Application of nereistoxin compound in prevention of marine biofouling

A technology of marine biofouling and nereistoxins, which is applied in the fields of compounds of group 5/15 elements of the periodic table, bismuth organic compounds, paints containing biocides, etc., can solve the problems of undiscovered nereistoxins and Application and other issues, to achieve the effect of mature artificial synthesis process, good application prospects, and environmental friendliness

Active Publication Date: 2017-05-31
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no reports and applications of nereistoxins have been found in the field of marine antifouling

Method used

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  • Application of nereistoxin compound in prevention of marine biofouling
  • Application of nereistoxin compound in prevention of marine biofouling
  • Application of nereistoxin compound in prevention of marine biofouling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Detection of Antifouling Activity of Nereistoxin Compounds on White Ridge Barnacle Venus Larvae

[0043] Barnacles belong to the Arthropoda phylum Crustacea Cirripedes, and are the most harmful organisms caused by fouling in the world. They mainly form juvenile barnacles through the attachment and metamorphosis of their Venus larvae, and then form fouling. At present, barnacle Venus larvae have been used as the biological model, which has become the longest, most widely used and most important antifouling detection activity model. In this embodiment, the larvae of the white-ridged barnacle Balanus albicostatus are selected as the antifouling activity detection model, and the effect of the compound on the attachment rate of the larvae is used as an indicator for evaluating the antifouling activity. Adults were collected from reefs and oyster culture racks in Wutong Sea Area, Xiamen City, and Venus larvae were cultured in a laboratory.

[0044] Using methanol a...

Embodiment 2

[0046] Example 2 Detection of Antifouling Activity of Nereistoxin Compounds on Mussels

[0047] Sand sieve shellfish Mytilopsis sallei is a typical alien invasive fouling shellfish, which is native to the tropical waters of Central America and belongs to the bivalve clam clamidae. It is often distributed in the inner bay waters with slow water flow. Carry out attached life, form a thicker fouling layer in marine facilities, cause huge economic losses to aquaculture and inland shipping. In this embodiment, the effect of the compound on the secretion number of sand sieve shellfish silk is used as the basis for evaluating its antifouling activity.

[0048] The shells of shellfish juveniles used in the experiment were about 10 mm long, and the surface was washed with sea water, and the original silk was cut off. Weigh nereistoxin and monosultap, and use dimethyl sulfoxide (DMSO) as solvent to prepare a series of concentration gradient solutions respectively. Take a clean 24-well...

Embodiment 3

[0050] Example 3 Toxicity detection of nereistoxins to non-target organism Artemia

[0051] Artemia salina is one of the important groups of marine zooplankton, and it is also an ideal bait for larvae and juvenile shrimp, and is often used as a detection organism for toxicity tests. In this embodiment, the 24-h acute toxicity of nereistoxin compounds on Artemia larvae is detected, and the mortality rate of the larvae is used as the basis for evaluating the toxicity. Artemia eggs were added to membrane-filtered seawater, aerated, and hatched for 24 hours at 25°C under 3000-4000 Lux light conditions. After 24 hours, stage I nauplii were collected, and the larvae were added to fresh membrane-filtered seawater, aerated, and cultured in a dark environment at 25°C for 24 hours. After 24 hours, collect II-III stage larvae as experimental materials.

[0052] Using DMSO as solvent, prepare a series of solutions of nereistoxin and monosultap with concentration gradient. Using a 24-we...

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Abstract

The invention discloses the application of a nereistoxin compound in prevention of marine biofouling, and relates to the nereistoxin compound. The nereistoxin compound can significantly inhibit the attachment of a marine biofouling organism namely barnacles and inhibit shellfishes from generating byssi, has an efficient antifouling activity, and has low toxicity to a non-target organism namely fairy shrimps at the same time. The nereistoxin compound is obtained through biomimetic synthesis of natural organic compounds, is easy to degrade, is environment-friendly, and is high in safety; moreover, a synthetic process of the nereistoxin compound is mature, so that the nereistoxin compound is suitable for large-scale production, and has a great potential for scale application to marine antifouling paints.

Description

technical field [0001] The present invention relates to nereistin compounds, in particular to the application of nereistin compounds in preventing marine biological fouling. Background technique [0002] Marine fouling organisms refer to the general term for microorganisms, plants and animals that inhabit or attach to the surface of ships and various underwater artificial facilities in the marine environment. Marine fouling organisms have brought huge threats to human economic activities and environmental ecology, such as increased ship navigation resistance and energy consumption, increased greenhouse gas emissions, reduced facility performance, accelerated metal corrosion, and alien biological invasion. Destroy the ecological balance and even lead to ecological disasters. In the technical development process of preventing marine fouling organisms from attaching and growing (anti-fouling), the use of chemical anti-fouling coatings is the most economical, effective and comm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D339/04C07D339/08C07D341/00C07C327/22C07C331/04C07F9/165C07F9/94C07D495/10C07D409/04C07D498/10C09D5/16
CPCC07C327/22C07C331/04C07D339/04C07D339/08C07D341/00C07D409/04C07D495/10C07D498/10C07F9/1651C07F9/94C09D5/1625
Inventor 刘辉冯丹青陈思羽盛彦青黄燕秋苏培何健柯才焕
Owner XIAMEN UNIV
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