A kind of all-male breeding method of asparagus

An asparagus and tetraploid technology is applied in the field of all-male breeding of asparagus, which can solve the problems of low viability and low haploid induction rate, and achieve the effects of strong viability, improved transplant survival rate, and improved induction efficiency.

Active Publication Date: 2019-02-01
河北省农林科学院经济作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the prior art, the anther culture of diploid plants has a very low haploid induction rate of less than 10%; and the haploid plants obtained by the anther culture of diploid plants have low viability due to the lack of half of the chromosomes , the colonization survival rate is only below 30%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Acquisition and planting of asparagus tetraploid plants: using the asparagus variety champion as the material, the seeds were sown after germination, and 40 days after sowing, the top of the tender stems of the seedlings was wrapped with absorbent cotton soaked in 0.1% colchicine solution for 48 hours, and then rinsed with water After culturing for another 10 days, the shoot tips were stained and pressed, and the number of chromosomes was observed under a microscope. Seedlings (tetraploid) with 40 chromosomes were screened out, and 62 tetraploid plants were obtained (80 plants were treated). 77.5%. Afterwards, the tetraploids were cultured for 30 days and planted in the field.

[0022] (2) Optimizing plants and flower buds: Select tetraploid single plants with excellent comprehensive characteristics as materials in the field. During the flowering period, before 10:00 in the morning, select flower buds from strong male single plants, and use tweezers to pull out smal...

Embodiment 2

[0031] (1) Acquisition and planting of asparagus tetraploid plants: using the asparagus variety champion as the material, the seeds were sown after germination. 30 days after sowing, the top of the tender stems of the seedlings was wrapped with absorbent cotton soaked in 0.12% colchicine solution for 50 hours, and then rinsed with water After culturing for another 8 days, take the shoot tips for staining and pressing, observe the number of chromosomes under a microscope, screen out seedlings (tetraploid) with 40 chromosomes, and obtain 58 tetraploid plants (80 plants for treatment), the induction rate 72.5%. Afterwards, the tetraploids were cultured for 40 days and planted in the field.

[0032] (2) Optimizing plants and flower buds: Select tetraploid single plants with excellent comprehensive characteristics as materials in the field. During the flowering period, before 10:00 in the morning, select flower buds from strong male single plants, and use tweezers to pull out small...

Embodiment 3

[0041] (1) Acquisition and planting of asparagus tetraploid plants: using the asparagus variety champion as the material, the seeds were sown after germination, and 35 days after sowing, the tops of the tender stems of the seedlings were wrapped with absorbent cotton soaked in 0.08% colchicine solution for 54 hours, and then rinsed with water After culturing for another 12 days, take the shoot tips for staining and pressing, observe the number of chromosomes under a microscope, screen out seedlings (tetraploid) with 40 chromosomes, and obtain 56 tetraploid plants (80 plants for treatment), the induction rate 70%. Afterwards, the tetraploids were cultured for 35 days and planted in the field.

[0042] (2) Optimizing plants and flower buds: Select tetraploid single plants with excellent comprehensive characteristics as materials in the field. During the flowering period, before 10:00 in the morning, select flower buds from strong male single plants, and use tweezers to pull out sm...

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Abstract

The invention relates to an asparagus holandric breeding method, belonging to the technical field of plant breeding. The method comprises the following steps: doubling chromosomes of a common diploid asparagus plant, thereby acquiring a tetraploid plant; performing anther culture on a tetraploid male plant, thereby acquiring a diploid super staminiferous plant; taking the super staminiferous plant as a male parent and excellent female plant as a female parent and performing hybridization assembling, thereby acquiring F1 generation holandric hybridized variety. The method is utilized to breed the asparagus holandric variety, the operability is high, the efficiency is high and the result is reliable.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for all-male breeding of asparagus. Background technique [0002] In the prior art, the anther culture of diploid plants has a very low haploid induction rate of less than 10%; and the haploid plants obtained by the anther culture of diploid plants have low viability due to the lack of half of the chromosomes , the colonization survival rate is only below 30%. Contents of the invention [0003] The object of the present invention is to provide a method for all-male breeding of asparagus aiming at the deficiencies of the prior art. In the method, chromosome doubling is first performed on diploid asparagus plants to obtain tetraploid materials, and then anther culture is carried out on tetraploid male materials to obtain diploid supermale plants, which greatly improves the induction rate of supermale plants, and simultaneously obtains The diploid sup...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01H4/00A01H1/02
Inventor 曹岩坡代鹏戴素英任文来
Owner 河北省农林科学院经济作物研究所
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