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Bacterium LRP3 capable of mineralizing and fixing heavy metal ions and application of bacterium LRP3

A technology of heavy metal ions and heavy metals, which is applied in the direction of bacteria, chemical instruments and methods, and the restoration of polluted soil, which can solve the problems of high cost, unstable effect of heavy metal polluted soil, easy secondary pollution, etc.

Inactive Publication Date: 2017-05-24
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of bacteria LRP3 capable of mineralizing and fixing heavy metal ions and its application in order to solve the problems of unstable effect, high cost and easy secondary pollution in the remediation process of heavy metal contaminated soil

Method used

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  • Bacterium LRP3 capable of mineralizing and fixing heavy metal ions and application of bacterium LRP3
  • Bacterium LRP3 capable of mineralizing and fixing heavy metal ions and application of bacterium LRP3
  • Bacterium LRP3 capable of mineralizing and fixing heavy metal ions and application of bacterium LRP3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of LRP3 Bacteria Fermentation Broth

[0044] First, the bacterial cells stored in the refrigerator at 4°C were inoculated into beef extract peptone medium and cultured for 24 hours, the bacterial cells were collected by centrifugation, and suspended in sterile water to prepare 10 7 -10 8 cells / mL of bacterial suspension. Then, according to the inoculum amount of 2%, the bacterial suspension was added to the above-mentioned culture medium and cultured at 25° C. and 160 rpm for 12-15 hours to obtain the LRP3 bacterial fermentation broth. The composition of the medium per 100mL is: sodium phytate 0.15-0.2g, beef extract 0.2-0.3g, peptone 0.3-0.5g, sodium chloride 0.3-0.5g, the balance is water, and the pH is 7.0-7.2 .

Embodiment 2

[0046] An experiment of removing heavy metal ions in aquaculture wastewater using bacteria LRP3 of the present invention.

[0047] Collect the aquaculture wastewater discharged from a pig farm in Changchun City after solid-liquid separation, take 20mL of wastewater and add it to 40mL of the bacterial LRP3 fermentation broth prepared in Example 1, place it statically for 1h, centrifuge at 8000rpm for 8-10min, take an appropriate amount of Save the supernatant. The collected raw wastewater was centrifuged under the same conditions, and the supernatant was saved. At the same time, the heavy metal ions in the wastewater before and after mineralization and consolidation are measured, and the removal rate of heavy metals is calculated. The test results show that the removal rates of Cu, Zn, Pb and Cd ions in wastewater are 92%, 91%, 87% and 92% respectively. It shows that the strain LRP3 can efficiently and quickly remove heavy metal ions in the water phase through adsorption, bio...

Embodiment 3

[0049] The experiment of mineralizing and immobilizing available heavy metals in soil using the bacteria LRP3 of the present invention and the product stability research.

[0050] In a facility vegetable planting base where livestock and poultry manure and organic fertilizers have been used for a long time in Jilin Province, the research on the mineralization and immobilization of the effective heavy metals in the soil by the bacteria LRP3 of the present invention is carried out. Heavy metal elements are Cu, Zn, Pb and Cd.

[0051] The bacterium LRP3 fermented liquid prepared by embodiment 1 is evenly sprayed fermented liquid in the soil of surface layer 0-20cm with the molar ratio of the phosphate radical ion in the bacterial agent and the effective state heavy metal in the 0-20cm surface layer soil of 1:1.5. Collect soil samples on the 3rd day after mineralization to measure the amount of available heavy metals, and calculate the reduction rate of available heavy metals. The...

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Abstract

The invention provides a bacterium LRP3 capable of mineralizing and fixing heavy metal ions and application of the bacterium LRP3. The bacterium LRP3 capable of mineralizing and fixing heavy metal ions provided by the invention is Rahnella sp. which is preserved in the general microbial center of China Microbial Culture Collection Committee and has a preservation number of CGMCC NO.13347. The bacterium can separate out an indigenous microorganism capable of degrading phytic acid by secreting phytase and alkaline phosphatase to release phosphate radicals from the environment; a fermentation product can rapidly precipitate and consolidate heavy metal ions in soil and aqueous solution through bio-mineralization so as to form a mineralization product with stable properties; the bacterium can also be planted in soil to enhance a natural mineralization process of heavy metals in the soil; a fermented liquid of the bacterium can be used for controlling the soil or wastewater polluted by the heavy metals to achieve simple operation, low cost and no secondary pollution.

Description

technical field [0001] The invention belongs to the technical field of applied environmental microorganisms, and in particular relates to a bacteria LRP3 capable of rapidly fixing heavy metal ions through biomineralization, and its application in treating heavy metal polluted environments. Background technique [0002] Due to sewage irrigation, industrial production wastewater pollution and the extensive use of organic fertilizers with high heavy metal content, the content of heavy metals in soil has gradually increased, and has become an important class of pollutants that seriously affect the quality of soil and water environments. Heavy metals cannot be degraded in the environment, and can be retained in soil and water through various physical, biological and chemical processes, and their activity will change as environmental conditions change. Therefore, heavy metals pollute the environment Governance has the disadvantages of difficulty and repetition. The current contro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/20C02F101/22
CPCB09C1/10C02F3/34C02F2101/20C02F2101/22C12N1/205C12R2001/01
Inventor 李明堂张欢焉莉赵兴敏李哲冷粟吴迪
Owner JILIN AGRICULTURAL UNIV
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