Lotion for fading scar
A kind of lotion and scar reduction technology, applied in the field of skin care products, can solve the problems of scar removal and scar reduction skin care products, and achieve the effect of restoring normal skin tone, lightening scars, and maintaining metabolism
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Embodiment 1
[0038] (1) Mangosteen isolated material preparation: collect fresh branches of mangosteen, clean, cut into 6cm, soak in 50mg / L L-ascorbic acid for 6min; put into 70% ethanol solution and soak for 5min, rinse with sterile water; put into 32 % hydrogen peroxide solution for 11 minutes, and then rinsed with sterile water for 12 minutes.
[0039] (2) Culture to obtain cambium cells: remove the phloem of mangosteen in vitro material, put it into MS solid medium containing 0.6 mg / L NAA, 3 mg / LBA and 1.2 mg / L hydrolyzed casein for dark culture, and the culture temperature is 26 °C , the cultivation humidity was 70%, and the cultivation time was 18 days.
[0040] (3) Subculture: Inoculate the cambium cells in MS liquid medium containing 1.2mg / L 2,4-D, 1.5mg / LBA, 1.5mg / L hydrolyzed casein and 1.5mg / L activated carbon in a shaker dark Cultivate under the following conditions: temperature 26°C, rotation speed 100r / min, time 16d, glass beads can be added to MS liquid medium.
[0041] (4...
Embodiment 2
[0044](1) Preparation of mangosteen isolated material: collect fresh branches of mangosteen, clean, cut into 7cm, soak in 75mg / L L-ascorbic acid for 7.5min; put into 70% ethanol solution and soak for 6.5min and rinse with sterile water; Soak in 36% hydrogen peroxide aqueous solution for 13 minutes, then rinse with sterile water for 13.5 minutes.
[0045] (2) Culture to obtain cambium cells: take off the phloem of mangosteen in vitro material, put into MS solid medium containing 0.9mg / LNAA, 3.5mg / LBA and 1.4mg / L hydrolyzed casein for dark culture, and the culture temperature is 29 ℃, the cultivation humidity is 75%, and the cultivation time is 21 days.
[0046] (3) Subculture: inoculate the cambium cells in MS liquid medium containing 1.4mg / L 2,4-D, 1.75mg / LBA, 1.75mg / L hydrolyzed casein and 1.75mg / L activated carbon in a shaker dark The culture conditions are as follows: temperature 29°C, rotation speed 110r / min, time 18d, glass beads can be added to MS liquid medium.
[004...
Embodiment 3
[0050] (1) Preparation of mangosteen isolated material: collect fresh branches of mangosteen, clean, cut into 8cm, soak in 100mg / L L-ascorbic acid for 9min; put in 70% ethanol solution and soak for 8min, rinse with sterile water; put in 40 % hydrogen peroxide aqueous solution for 15 minutes, and then rinsed with sterile water for 15 minutes.
[0051] (2) Obtaining cambium cells by culturing: remove the phloem of mangosteen in vitro material, put it into MS solid medium containing 1.2 mg / L NAA, 4 mg / LBA and 1.6 mg / L hydrolyzed casein for dark culture, and the culture temperature is 32 °C , the cultivation humidity was 80%, and the cultivation time was 25 days.
[0052] (3) Subculture: Inoculate cambium cells in MS liquid medium containing 1.6mg / L 2,4-D, 2.0mg / LBA, 2.0mg / L hydrolyzed casein and 2.0mg / L activated carbon in the dark on a shaker Cultivation, the culture conditions are: temperature 32°C, rotation speed 120r, min, time 20d, glass beads can be added to MS liquid medi...
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