A kind of aseptic grafting method of Arabidopsis thaliana/salina mustard

Abstract

The invention discloses a sterile grafting method of Arabidopsis / thellungiella halophila and fills up the technical blank of related grafting methods in the prior art. According to the technical scheme, the method includes: using a sterile thellungiella halophila seedling 7-10 days after germination as the stock, and using the overground part of a sterile Arabidopsis seedling 3-5 days after germination as the scion; grafting under a sterile condition, placing the grafted seedling on a 1 / 2MS culture medium with agar powder concentration being 1.2%, performing vertical culture under 18-22 DEG C and short-day illumination, and transferring the grafted seedling into a 1 / 2hogland nutrient solution for water culture or nutrient soil culture. The sterile grafting method has the advantages that the method is simple and practicable, convenient to operate, capable of reducing pollution, high in survival rate, low in damage and low in cost; the sterile grafting method for different species provides a new thought for the varietal improvement of gardening, fruits, vegetables, and the like, and a basic guarantee is provided for plant grafting in plant molecular mechanism researches.

Description

technical field [0001] The invention relates to the technical field of grafting of small plant test-tube seedlings, in particular to a method for aseptic grafting between the model plant Arabidopsis thaliana and the salt-tolerant model plant Saltina thaliana. Background technique [0002] Grafting is a kind of vegetative reproduction in asexual reproduction, which is carried out by utilizing the wound healing function of plants. As an agricultural production technology, it has been widely used in agricultural, forestry and horticultural plant production and scientific research and development. Since the beginning of the last century, especially in the 1970s and 1980s, people have done a lot of research on the basic theory of grafting. However, some mechanism issues are still unclear. [0003] Arabidopsis thaliana is a small weed in the Brassicaceae family. Because of its short growth period, small individual size, and small genome, it has long been used as a model plant fo...

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Problems solved by technology

Moreover, the graft survival rate of different species in different periods is also different, even the same species in different periods, the survival rate of the graft body is also very different

[0006] In summary, there are many factors that affect the survival of grafting. As two plants of the same family and different genera, Arabidopsis thaliana and Salina thaliana are more difficult to graft, and there is no research on the grafting methods of Arabidopsis and Salina thaliana. report, still technically blank

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