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Treatment method of mycoplasma contamination

A treatment method and technology for mycoplasma, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve the problems of cell metabolism and function changes, hidden safety hazards of biological products, and inability to use, to achieve simple operation and improve cell viability. , the effect of lowering the application threshold

Inactive Publication Date: 2017-05-10
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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Problems solved by technology

Cells contaminated by mycoplasma will inevitably change in cell metabolism and function, thus affecting the results of experiments using contaminated cells, and it is also a safety hazard for biological products
[0004] At present, once the cultured cells are contaminated with mycoplasma, most of the solutions are inactivated and discarded, but if the cells are very precious and cannot be replaced, this method cannot be used

Method used

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  • Treatment method of mycoplasma contamination
  • Treatment method of mycoplasma contamination
  • Treatment method of mycoplasma contamination

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Embodiment Construction

[0038] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0039] see Figure 1-Figure 2 , a kind of processing method of mycoplasma pollution of the present invention is described in detail:

[0040] (1) Obtain the cells to be treated.

[0041] Specifically, the method for obtaining cells to be treated includes the following steps:

[0042] Culture mycoplasma-contaminated cells in a culture flask to a second preset density, discard the supernatant, and wash twice with phosphate buffer solution;

[0043] Digestion with trypsin solution;

[0044] Stop the digestion with fresh basal medium and...

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Abstract

The invention discloses a treatment method of mycoplasma contamination. The treatment method comprises the following steps: obtaining cells to be treated; culturing the cells to be treated by utilizing a culture medium containing 10mu.g / mL of ciprofloxacin; updating the culture medium at fixed time, and enabling the growth density of the cells to be treated to be kept at 50 percent to 80 percent; stopping utilizing the culture medium until no mycoplasma contamination condition is detected. According to the treatment method of the mycoplasma contamination, disclosed by the invention, mycoplasmas are removed by adopting a method of adding the ciprofloxacin into the culture medium and the aim that the mycoplasma contamination is completely removed under the condition that influences on the cells are small is realized, without the need of adopting a manner of inactivating firstly and discarding secondly; meanwhile, the treatment method also has the advantage that the mycoplasma contamination does not reoccur after the ciprofloxacin is not used.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, to a treatment method for mycoplasma pollution. Background technique [0002] Cell culture technology is a routine experimental technique for proliferating cells in vitro, and is currently widely used in teaching, scientific research and clinical experiments. Contamination control is critical to the success of cell culture. Microorganisms that cause contamination of cultured cells include bacteria, molds, black colloids, and mycoplasma, among which mycoplasma is a common pollutant. [0003] Mycoplasma is smaller than bacteria and fungi in size, and its existence cannot be observed with a general optical microscope, and a considerable part of cells contaminated by mycoplasma can still grow and proliferate, so mycoplasma pollution is not easy to find compared to bacteria and fungi. . Cells contaminated by mycoplasma will inevitably change cell metabolism and function, which will a...

Claims

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Application Information

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IPC IPC(8): C12N5/00
CPCC12N5/00
Inventor 高毅傅超毅李阳彭青
Owner ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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