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Extracellular ratio-type oxygen sensing probe, and preparation method and applications thereof

An oxygen sensing probe, ratiometric technology, applied in the field of extracellular ratiometric oxygen sensing probe and its preparation, can solve problems such as low quantum efficiency

Active Publication Date: 2017-05-10
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some hydrophilic oxygen probes prepared from metalloporphyrins (PtCPK, PtCP, PdCPK, or PtTCPP) exhibit extremely low quantum efficiencies ranging from 0.001 to 0.0095

Method used

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  • Extracellular ratio-type oxygen sensing probe, and preparation method and applications thereof
  • Extracellular ratio-type oxygen sensing probe, and preparation method and applications thereof
  • Extracellular ratio-type oxygen sensing probe, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] In this example, the polymer carrier shown in formula a was synthesized through the following process:

[0123]

[0124] where A is

[0125] The synthesis steps are as follows:

[0126] P1 synthesis process:

[0127] 988mg (8.7mmol) ε-caprolactone and 61.1mg (0.1mmol) aggregation-induced luminescent molecule A-OH (synthesized according to the following literature: Hongguang Lu, et al, JOURNAL OF POLYMER SCIENCE PART A: POLYMERCHEMISTRY 2012,50,890– 899) was fully mixed in the flask, and stirred in an oil bath at 110°C under a nitrogen atmosphere for 5 minutes, and then injected two drops of catalyst stannous octoate (Sn(Oct) 2 ), after stirring and reacting overnight, the reactant was rapidly cooled in an ice bath to obtain a solid polymer. Afterwards, the solid polymer was redissolved in 5 mL of dichloromethane, and then the solution was slowly added dropwise to 100 mL of ice methanol to obtain a polymer precipitation, and the precipitation process was repeated...

Embodiment 2

[0133] In this example, the polymer carrier in formula d is prepared by the following reaction

[0134]

[0135] 73mg (0.06mmol) P2, 440mg (0.8mmol) oligoethylene glycol methyl ether methacrylate (OEGMA, molecular weight 550), 10mg AIBN were placed in a 10mL Schlenk tube, and filled with nitrogen after using the traditional deoxygenation method The gas was exchanged three times, heated to 70°C and stirred, and reacted for 24 hours. After the reaction was completed, it was added dropwise into glacial ether to obtain viscous polymer P4. Yield 84%. Mn=35400, Mw=43200. x:y=7.5:72.5.

Embodiment 3

[0137] The ability of the polymer carrier prepared in Example 1 to form micelles in an aqueous solution is investigated by measuring its critical micelle concentration. During the formation of micelles, the fluorescence emission will be quenched by water to obtain a weaker micelle concentration. fluorescence. Once micelles are formed, the fluorescence intensity will be greatly enhanced. The CMC of the polymer carrier prepared in Example 1 was judged by measuring the change of the fluorescence intensity of P3 at a concentration of 0.01 μg / mL to 100 μg / mL in an aqueous solution, and judging the sudden change point of the fluorescence intensity. During the test process, the fluorescence intensity changes with the concentration as figure 1 As shown, the visible nanosystem has a strong absorption at a wavelength of 530 nanometers, and the fluorescence intensity increases with the increase of the polymer concentration. The concentration and the fluorescence intensity value at 530n...

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Abstract

The invention provides an extracellular ratio-type oxygen sensing probe, and a preparation method and applications thereof. The ratio-type oxygen sensing probe comprises a carrier and an oxygen sensitive compound, which is wrapped in the carrier. The carrier is composed of an amphiphilic graft polymer and aggregation-induced luminous groups connected to the amphiphilic graft polymer. The provided ratio-type oxygen sensing probe can measure the oxygen consumption amounts of escherichia coli and GM12878 human B lymphocyte under different cell concentrations; at the same time, can in-situ measure at least 100 cfu / mL of cells within 7 hours, can also applied to drug screening, does not have any cytotoxicity, and is safe and reliable. Furthermore, due to the extremely high quantum efficiency of PtTFPP probe micelles, the oxygen sensing probe can be used to measure the oxygen content of water solution; and the probe can be widely applied to commercial instruments such as ELISA reader, and assists the high throughput measurement, disease diagnosis, metabolism research, and the like.

Description

technical field [0001] The invention belongs to the field of nano medical materials, and relates to a ratio-type oxygen sensing probe and its preparation method and application, in particular to an extracellular ratio-type oxygen sensing probe and its preparation method and application. Background technique [0002] Oxygen is a very important element for the environment, ocean, agriculture, industry, health, etc. In the biological field, oxygen, as a key basic substance in aerobic cell nuclear organization, plays an extremely important role in many core cell physiology and developmental processes including energy production, energy balance, and biomass generation. As an electron acceptor, oxygen is critical for electron transfer (ETC) functions in mitochondria and ATP production. Oxygen availability and / or availability, over time, contributes to pathology and disease states and thus represents an important goal of medical diagnostics and basic research. For example, it has...

Claims

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Application Information

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IPC IPC(8): C09K11/06C09K11/02C08F290/06C08F220/58C08F220/28G01N21/64
CPCG01N21/6402G01N21/6486C09K11/02C09K11/06C08F290/061C09K2211/185C08F220/286C08F220/58
Inventor 田颜清邹贤劭潘婷婷蒋嘉培
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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