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Polyacrylamide gel preparation method capable of preventing solution leakage

A technology of polyacrylamide gel and solution, which is applied in the direction of instruments, measuring devices, scientific instruments, etc. It can solve the problems of the bottom of the two glass plates not being tightly sealed, the acrylamide solution leaking, and the assembly of the glass plates not in place, etc., to ensure The effect of agarose gel activity, strong practicability, and less facilities

Active Publication Date: 2017-04-19
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

However, in the process of making glue, often due to various reasons, such as outdated equipment, improper assembly of glass plates, etc., the bottom of the two glass plates is not tightly sealed, causing the acrylamide solution to leak from the bottom of the glass plate before it solidifies into a gel. Glue failed

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  • Polyacrylamide gel preparation method capable of preventing solution leakage

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Embodiment 1

[0021] In this embodiment, a method for preparing a polyacrylamide gel to prevent solution leakage, figure 1 Shown are the rubber-making equipment, equipment and experimental conditions built in this embodiment.

[0022] Equipment: ①Pipette gun with a volume of 1mL, ②1mL tip, ③1% agarose gel solution, ④Assembled glass plate for preparing polyacrylamide gel.

[0023] A constant temperature shaking incubator is used as an incubator, and the temperature is adjusted to 50°C, but its shaking function is not used.

[0024] During the operation, first take out ①~④ that have been preheated at 50°C, that is, a pipette with a volume of 1mL, a 1mL tip, 1% agarose gel solution, and a glass plate, and operate at room temperature.

[0025] First, install the tip of the pipette with one hand and draw 400 μL of 1% agarose solution, hold the front glass 1 and back glass 2 horizontally with the other hand, and then align the tip of the pipette with the front glass 1 and the back glass. Quickl...

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Abstract

The invention relates to a polyacrylamide gel preparation method capable of preventing solution leakage, and belongs to the technical field of physical or chemical apparatus. The bottoms of two pieces of glass are sealed by agarose gel; two pieces of glass are placed on two tables with a same height, two ends, which are connected to each table, of each glass are horizontal, a gap exists between the lower end of each glass and the surface of an operation table, a gel forming area is formed in the gap above the bottoms of two pieces of glass, and gel forming raw materials are added into the gel forming area to start gel production. The preparation method is used to prepare polyacrylamide gel, has the advantages of simpleness, convenience, and rapidness, and is capable of reducing solution waste and guaranteeing the number of gel blocks.

Description

technical field [0001] The invention relates to a polyacrylamide gel preparation method for preventing solution leakage, and belongs to the technical field of physical or chemical devices. Background technique [0002] Polyacrylamide gel electrophoresis (gel electrophoresis), referred to as PAGE, is the most common and basic method for qualitative and quantitative analysis of a small amount of protein or nucleic acid in molecular biology research. Add sodium dodecyl sulfate (sodium dodecyl sulfate, SDS) to the gel, that is, SDS-PAGE, without adding SDS, it is Native PAGE. For general protein detection tests, currently laboratories generally use Shanghai Tianneng and Bio-Rad’s micro-vertical PAGE systems, and use glass plates and spacers to prevent the leakage of acrylamide solution when making glue. However, in the process of making glue, often due to various reasons, such as outdated equipment, improper assembly of glass plates, etc., the bottom of the two glass plates is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/44747
Inventor 杨仙玉宋源普李玉兰蒋桂瑾贾宇坤刘怡君
Owner ZHEJIANG FORESTRY UNIVERSITY
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