Pilose antler deep processing method
A technology of deep processing and velvet antler, which is applied in the fields of medical formula, food science, and medical preparations containing active ingredients, etc. It can solve the problems of velvet antler bone powder waste, inability to obtain nutrients, absorption and utilization, etc., and achieve convenient consumption, easy absorption, and reaction conditions mild effect
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Embodiment 1
[0017] Using fresh sika deer antler as raw material, it is prepared according to the following process:
[0018] Crush 1 kg of velvet antler into 100-mesh powder, add 1 liter of pH 2 phosphate buffer, stir evenly, add 10 grams of velvet antler pepsin, and enzymatically hydrolyze at 20°C for 0.5 hours. After enzymatic hydrolysis, the temperature was raised to 80°C and kept for 10 minutes. The enzymolysis solution was centrifuged at a speed of 1000 rpm for 10 minutes; the supernatant was freeze-dried to obtain deboned antler extract, and the precipitate was freeze-dried to obtain antler bone powder.
[0019] Take 50g of dry phospholipid and cholesterol mixture, the mass ratio of phospholipid and cholesterol is 1:1, dissolve the mixture in 5 liters of chloroform and mix well, then vacuum rotary evaporation at 30℃ to form a lipid film. Hydrate the lipid film with 10 liters of deionized water, add glass beads with a diameter of 0.5 to 3 mm, stir at 30°C for 20 minutes, and then st...
Embodiment 2
[0022] Using fresh red deer antler as raw material, it is prepared according to the following process:
[0023] Crush 100 grams of deer antler into 500-mesh powder, add 1 liter of pH 7 phosphate buffer to dissolve, stir evenly, add 5 grams of papain, and enzymatically hydrolyze at 80°C for 24 hours. After the enzymatic hydrolysis, the temperature was raised to 100°C and kept for 60 minutes. The enzymolysis solution was centrifuged at a speed of 10,000 rpm for 60 minutes; the supernatant was freeze-dried to obtain deboned velvet antler extract, and the precipitate was velvet antler bone powder after freeze-drying.
[0024] Take 50mg of dry phospholipid and cholesterol mixture, the mass ratio of phospholipid to cholesterol is 1:0.1, dissolve the mixture in 50ml of chloroform and mix well, then vacuum rotary evaporation at 80℃ to form a lipid film. Hydrate the lipid film with 500ml of deionized water, add glass beads with a diameter of 0.5-3mm, stir at 90°C for 60 minutes, and t...
Embodiment 3
[0027] Using dried sika deer antler as raw material, it is prepared according to the following process:
[0028] Crush 100 grams of velvet antler into 300-mesh powder, add 500 ml of pH 6 phosphate buffer to dissolve, stir evenly, add 10 grams of velvet antler trypsin, and enzymatically hydrolyze at 40°C for 5 hours. After enzymatic hydrolysis, the temperature was raised to 80°C and kept for 20 minutes. The enzymolysis solution was centrifuged at a speed of 5000 rpm for 20 minutes; the supernatant was freeze-dried to obtain deboned antler extract, and the precipitate was freeze-dried to obtain antler bone powder.
[0029] Take 50mg of dry phospholipid and cholesterol mixture, the mass ratio of phospholipid and cholesterol is 1:0.01, dissolve the mixture in 1000ml of chloroform and mix well, then evaporate in vacuum at 40℃ to form a layer of lipid film. Add 500ml of deionized water to hydrate the lipid film, add glass beads with a diameter of 0.5-3mm, stir at 90°C for 60 minute...
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