Inverse probe for visually detecting single-nucleotide polymorphism site in gene sequence, kit and detection method thereof

Abstract

The invention discloses an inverse probe for visually detecting a single-nucleotide polymorphism site in a gene sequence, a kit and a detection method thereof. The inverse probe comprises a sequence H1, a sequence H2, a sequence P1 and a G-four strobile structure; the sequence H1 is an inverse complementary sequence at the end of a sequence SNP site 5' to be detected; the sequence H2 is an inverse complementary sequence at the end of the sequence SNP site 3' to be detected; the sequence P1 is an inverse complementary sequence of a universal primer of the sequence to be detected; and the G-four strobile structure is an inverse complementary sequence of a G-four strobile gene of the sequence to be detected. According to the inverse probe disclosed by the invention, the result is simple and synthesis is convenient; the detection of the SNP site can be completed in a single PCR test tube; a detection result shows that the result can be judged via color observation with eyes; according to the detection method, the operation is easy, the detection is convenient, the use cost is low and the detection method is suitable for outdoor, field and site direct detection and species identification, so that statistic analysis of population heredity is realized.

Description

Technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a reverse probe, a kit and a detection method for visually detecting single nucleotide polymorphism sites in a gene sequence. Background technique [0002] Single Nucleotide Polymorphism (SNP) is a polymorphism in the nucleic acid sequence caused by the change of a single nucleotide base in the genome. It is a nucleoside on the same chromosome or at the same site in different individuals. In acid sequences, most of the nucleotide sequences are identical but only one base is different. There are 3 million SNPs in the human genome, with an average of about one SNP site for every 21,250 base pairs. Studies have shown that SNPs are related to the occurrence of many diseases, and rapid and gradual detection of these biologically significant SNPs is the basis and prerequisite for accurate medical diagnosis. On the one hand, SNP detection can refer to the existing geno...

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Problems solved by technology

[0004] These methods have their own advantages, such as simple operation, high detection specificity, high throughput or high precision, etc., but they all have special requirements and high dependence on experimental instruments, time-consuming detection and cumbersome operation, which limits other methods. Flexibility and scope of application

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