Nucleic-acid extraction, amplification and detection integrated device and manufacturing method and detection method thereof

Abstract

The invention discloses a nucleic-acid extraction, amplification and detection integrated device and a manufacturing method and a detection method thereof. The nucleic-acid extraction, amplification and detection integrated device comprises a light-cured-resin upper cover printed by the 3D printing technology and a base; the upper cover comprises a sample feeding hole, a control button, a U-shaped clamping fixing hole and a test paper result observation window; an integrated module on the base comprises a paper-base nucleic acid extraction module, an isothermal amplification module and a side-flow test paper detection module; the paper-base nucleic acid extraction module comprises a liquid storage tank, a pyrolysis channel, a washing channel, nucleic acid adsorption paper and a water absorption pad; the isothermal amplification module comprises a heating slice, a U-shaped clamp, a button battery and a PMMA plastic plate; a test-paper detection module comprises a test paper strip and a chromatographic solution storage tank. Finally, the upper cover is pressed on the base, and device assembling is completed. According to the paper-base nucleic acid extraction, amplification and detection integrated device, external device and professional technical persons are not required, and the paper-base nucleic acid extraction, amplification and detection integrated device has the advantages of being low in cost, easy to operate, rapid, easy to carry and the like, and is suitable for the field of molecular diagnosis, and particularly is suitable for resource-lack remote areas.

Description

[0001] 【Technical field】 [0002] The invention relates to an integrated device for nucleic acid extraction, amplification and detection, a manufacturing method and a detection method thereof. [0003] 【Background technique】 [0004] As a molecular marker, nucleic acid has been widely used in the fields of disease diagnosis, food safety detection and environmental monitoring. Nucleic acid-based diagnosis generally includes three parts: extraction, amplification and detection of nucleic acid. The traditional method mainly relies on large-scale instruments and professional technicians in the laboratory, which has disadvantages such as high cost, time-consuming and labor-intensive. In remote areas where infectious diseases are frequent, food safety problems are prominent, and environmental pollution is serious, these traditional methods are not applicable, which seriously delays the diagnosis and treatment of diseases, food testing, and environmental monitoring and control of peo...

AI Technical Summary

Problems solved by technology

They all require complex extraction and amplification operations, and also rely on large-scale instruments in the laboratory (such as thermal cyclers, refrigerators), and still have defects such as inconvenient portability, complicated operations, and high cost.

Fast, simple and portable nucleic acid detection cannot be realized

Still not suitable for molecular diagnostics in low-resource settings

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