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Method for preparation of composite microbial paraffin removal and inhibition agent

A technology for removing wax and preventing microorganisms, which is applied to the methods of using microorganisms, chemical instruments and methods, compositions for drilling holes, etc., can solve the problems of poor wax removal and preventing effect, etc., and achieves good effects, simple preparation steps and low cost. Effect

Inactive Publication Date: 2017-03-22
周荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: when the existing microbial wax removing and preventing agents are applied to high-temperature (above 90°C) high-salt (more than 15% salinity) oil wells, the effect of removing wax and preventing them is poor, which cannot meet the needs of oil fields. The problem of actual production requirements, the present invention will first use salted fish as raw material, ferment to obtain halophilic bacteria liquid, then use biogas liquid as raw material, after filtering, put the filtrate in self-made culture medium for cultivation, and obtain thermophilic bacteria Then inoculate it and halophilic bacteria liquid into the self-made acclimation medium for repeated culture to obtain mixed colonies of paraffin medium, cultivate them to obtain mixed bacteria, and finally inoculate them with substances such as sodium petroleum sulfonate After mixing, the microbial compound wax remover and preventive agent can be obtained. The microbial compound wax remover and preventive agent prepared by the present invention has a better effect of removing wax in oil wells with high temperature (above 90°C) and high salt (salinity above 15%) , enough to meet the actual production requirements of the oil field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] First weigh 30g of salted fish and add it to the fermenter, then add a 15% sodium chloride solution to submerge the salted fish, and ferment it at 45°C for 6 days. After the fermentation is over, collect the fermented liquid and filter it. Halobacteria liquid, for later use; weigh 30g of glucose, 15g of yeast extract, 8g of sodium chloride and 1000mL of deionized water, add them to a petri dish, stir and mix, transfer to a sterilizing tank, and sterilize at 105°C for 10 minutes. Prepare the culture medium, then take the biogas liquid for filtration, collect the filtrate, take 200mL of the filtrate and add it to the culture medium, and place it in a shaker, cultivate it at 150r / min, 85°C for 3h, remove the bacteria, and then lower the temperature to 75°C ℃, cultured for 3 days, after the culture was completed, collected the culture medium and placed it in a centrifuge, centrifuged at 1000r / min for 30min, collected the strains of the lower layer, and obtained the strains o...

example 2

[0021] First weigh 20g of salted fish and add it to the fermenter, then add a 15% sodium chloride solution to submerge the salted fish, and ferment for 5 days at 35°C. After the fermentation is over, collect the fermentation broth and filter it, and collect the filtrate to obtain Halobacteria liquid, for later use; weigh 20g of glucose, 10g of yeast extract, 5g of sodium chloride and 800mL of deionized water, add them to a petri dish, stir and mix, transfer to a sterilizing tank, and sterilize at 100°C for 5 minutes. Prepare the culture medium, then take the biogas liquid for filtration, collect the filtrate, take 100mL of the filtrate and add it to the culture medium, and place it in a shaker, and cultivate it at 130r / min, 80°C for 2h to remove miscellaneous bacteria, and then lower the temperature to 70°C Cultivate for 2 days at ℃. After the cultivation is over, collect the culture medium and put it in a centrifuge, centrifuge at 800r / min for 20min to collect the bacteria in ...

example 3

[0024] First weigh 25g of salted fish and add it to the fermenter, then add 15% sodium chloride solution to submerge the salted fish, and ferment for 5 days at 37°C. Halophilic bacteria liquid, for later use; weigh 25g glucose, 12g yeast extract, 7g sodium chloride and 900mL deionized water, add to a petri dish, stir and mix, transfer to a sterilizing tank, and sterilize at 102°C for 7 minutes, Prepare the culture medium, then take the biogas liquid for filtration, collect the filtrate, take 150mL of the filtrate and add it to the culture medium, put it in a shaker, and cultivate it at 140r / min, 82°C for 2h to remove miscellaneous bacteria, and then lower the temperature to 72°C ℃, culture for 2 days, after the culture is over, collect the culture medium and put it in a centrifuge, centrifuge at 900r / min for 25min, collect the bacteria in the lower layer, and get the thermophilic bacteria; then weigh 12g of beef extract and 12g of glucose , 25g of paraffin, and 250mL of deioni...

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PUM

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Abstract

The present invention relates to a method for preparation of a composite microbial paraffin removal and inhibition agent, and belongs to the technical field of preparation of a paraffin removal and inhibition agent. The method firstly uses salted fish as a raw material to be subjected to fermentation to obtain halophilic bacterium liquid. Then biogas liquid is used as a raw material to be subjected to filtration, and the obtained filtrate is cultured in a self-made medium to obtain thermophilic bacterium strains. And then a self-made domestication medium is inoculated with the thermophilic bacterium strains and the halophilic bacterium liquid, repeated culture is performed to obtain a paraffin medium mixed bacterial colony and the paraffin medium mixed bacterial colony is cultured to obtain a mixed bacterial strain; and finally, the mixed bacterial strain is mixed with materials such as petroleum sodium sulfonate and the like to obtain the composite microbial paraffin removal and inhibition agent. The prepared composite microbial paraffin removal and inhibition agent by the method provided by the present invention has a relatively good paraffin removal and inhibition effect in an oil well with the high temperature (90 DEG C or above) and high salt (salinity of 15 percent or above), and the speed of paraffin dissolution is higher than 127g / min.m<2>, and the ratio of paraffin inhibition is 0.032g / min or above. The composite microbial paraffin removal and inhibition agent does not have corrosivity and does not corrode tubing.

Description

technical field [0001] The invention relates to a preparation method of a microbial composite wax remover and preventive agent, and belongs to the technical field of preparation of wax remover and preventive agents. Background technique [0002] Wax deposition in oil wells will affect normal production, so wax removal and prevention in oil wells is also a problem that needs to be solved in oil recovery. The anti-wax technology is to add additives to the oil well to achieve the purpose of removing wax. Anti-wax mechanism: The process of wax deposition is mainly the growth process of crystals. If the formation of nuclei can be controlled, the growth of crystals can be blocked, and wax crystals cannot form in the end. [0003] At present, the wax removers and preventive agents used in oil fields mainly include oil-based wax removers and water-based wax removers. Due to the low flash point of oil-based wax remover and preventive agent, the wax remover and preventive agent used...

Claims

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Application Information

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IPC IPC(8): C09K8/524C12N1/36
CPCC09K8/524C12N1/36
Inventor 周荣薛荣飞孟中立
Owner 周荣
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