Composite type synbiotic microecological preparation for chickens and preparation method of composite type synbiotic microecological preparation

A kind of micro-ecological preparation and composite technology, applied in the direction of bacteria used in food preparation, application, animal feed molding or processing, etc., can solve the problems of reducing the quality of chicken and egg products, drug residues, etc., to improve immunity and Production performance, growth stimulation, production performance improvement effect

Inactive Publication Date: 2017-03-22
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a compound synbiotics microecological preparation for chickens and its preparation method, aiming to solve the problem that more and more pathogenic bact...

Method used

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  • Composite type synbiotic microecological preparation for chickens and preparation method of composite type synbiotic microecological preparation

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preparation example Construction

[0037] Such as figure 1 As shown, the preparation method of the compound synbiotic probiotics for chickens provided by the embodiments of the present invention comprises the following steps:

[0038] S101: Isolate and screen the probiotics of Lactobacillus, Bifidobacterium, and variable X bacteria from the gastrointestinal tract of chickens and the soil near the chicken farm, and analyze the probiotics of the isolated Lactobacillus, Bifidobacteria, Bacillus, and Enterococcus The morphological characteristics of bacteria and the identification of bacteria species;

[0039] S102: compare the biological characteristics, functions and safety identification of the isolated probiotics of Lactobacillus, Bifidobacterium, Bacillus and Enterococcus, evaluate the probiotic effect in vitro, and initially screen high-quality probiotic strains;

[0040] S103: Cultivate the preliminarily screened high-quality probiotics in the artificially simulated gastrointestinal fluid, measure the numbe...

Embodiment 1

[0048] Example 1: Preliminary screening of Enterococcus probiotics from soil in chicken farms.

[0049] Step 1: Collection of soil samples containing enterococci. Choose the soil of the chicken breeding place in a large farm as the collection destination, randomly select 3-5 small areas, take 20-40g of original soil from each area, divide it into 2 groups, put them into sterilized ziplock bags, and quickly bring them back to the experiment Store in a 4°C refrigerator for later use;

[0050] The second step: the isolation of enterococci. Under sterile conditions, the two groups of soil samples were added to the enriched culture solution to form colonies, and then the bacteria were inoculated into the bacterial suspension made of sterile normal saline, and then diluted into different strains by the 10-serial decreasing method. Inoculate the samples of different solubility gradients into the culture medium by streaking on the plate, make 3 parallels for each dilution, and put t...

Embodiment 2

[0054] Preliminary screening of Bacillus subtilis from chicken feed grains.

[0055] The first step: the collection of samples in the feed trough. Select each chicken breeding place as the sample collection destination, randomly select several feed troughs, take a sample of 20-30g from each place, put them into sterilized ziplock bags, bring them back to the laboratory and store them in a 4°C refrigerator for later use;

[0056] The second step: enrichment and preliminary isolation of Bacillus subtilis. Under aseptic conditions, the two groups of feed tank samples were added to the enriched culture solution to form colonies, and then the bacteria were inoculated into sterile normal saline to make a bacterial suspension, and then diluted by 10 serial decrement method to obtain For different concentration gradients, inoculate samples of different solubility gradients onto the culture medium by streaking on the plate, make 3 parallels for each dilution, and put the resulting pla...

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Abstract

The invention discloses a composite type synbiotic microecological preparation for chickens and a preparation method of the composite type synbiotic microecological preparation. The composite type synbiotic microecological preparation consists of probiotics and prebiotics, wherein the probiotics and the prebiotics are mixed according to the mass ratio of the probiotics to the prebiotics being 1 to (1-5); the probiotics consist of bacterium lacticum, bifidobacteria, bacillus and enterococcus; and the prebiotics consist of oligosaccharide and an acidulant, the oligosaccharide can be mannose oligosaccharides, fructo-oligosaccharide, isomaltooligosacharide and gynolactose, and the acidulant is a composite acidulant consisting of lactic acid, citric acid and fumaric acid. According to the composite type synbiotic microecological preparation and the preparation method thereof disclosed by the invention, the defect of separately using probiotic products for raising commodity chickens is overcome, besides, the composite type synbiotic microecological preparation is suitable for various commodity chickens, the purposes of making best use of the advantages and overcoming the disadvantages can be achieved, the field planting of exogenous probiotics in intestinal tracts of animals is promoted, the rapid growth and propagation of various probiotics in chicken bodies is selectively stimulated, the purpose of promoting the health of the chickens is achieved, the production properties are also greatly improved, the influence of conventional edible animal additives on chicken meat and egg quality is reduced, and the composite type synbiotic microecological preparation and the preparation method are economic and practical.

Description

technical field [0001] The invention belongs to the technical field of probiotics, and in particular relates to a compound synbiotic probiotics for chickens and a preparation method thereof. Background technique [0002] At present, there are few studies on the application of synbiotics microecological preparations in animal production in my country, and the use of microecological preparations in animal food additives in animal husbandry is still relatively small. People are vague about the application of probiotics, that is, confusion is targeted Sexually added objects, resulting in animal growth that is often less than expected. The emergence of probiotics is due to the improvement of people's living standards, and people gradually realize that antibiotics added to feed for a long time can cause harm to human health through the food chain. The research and application of microecological preparations overcomes the various disadvantages caused by the long-term use of antibio...

Claims

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Application Information

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IPC IPC(8): A23K50/75A23K10/18A23K20/163A23K20/105A23K40/00
CPCA23V2400/11A23V2400/51
Inventor 刘君刘华康王晗陈静罗雁刘永明张薇
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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