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Method for establishing fingerprint spectrum of Taohe Chengqi decoction composition and fingerprint spectrum

A technology of fingerprint spectrum and establishment method, applied in the field of traditional Chinese medicine analysis, can solve the problem of no Taohe Chengqi Decoction composition, etc., and achieve the effects of convenient operation, objective conclusion, and control of preparation quality

Active Publication Date: 2017-03-08
CHINA RESOURCES SANJIU MEDICAL & PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Therefore, the technical problem to be solved in the present invention is to overcome the defect in the prior art that there is no fingerprint of the Taohe Chengqi Decoction composition and the bibliographical report of the establishment of the fingerprint of the Taohe Chengqi Decoction composition, thereby providing a Method for establishing fingerprints of Taohe Chengqi Decoction composition and its fingerprints

Method used

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  • Method for establishing fingerprint spectrum of Taohe Chengqi decoction composition and fingerprint spectrum
  • Method for establishing fingerprint spectrum of Taohe Chengqi decoction composition and fingerprint spectrum
  • Method for establishing fingerprint spectrum of Taohe Chengqi decoction composition and fingerprint spectrum

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Experimental program
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Effect test

Embodiment 1

[0078] Embodiment 1. prepare reference substance solution

[0079] Aloe-emodin reference substance solution Take an appropriate amount of aloe-emodin reference substance, weigh it accurately, add methanol to make a solution containing 80 μg per 1 ml, and obtain it.

[0080] Rhein reference substance solution Take an appropriate amount of rhein reference substance, weigh it accurately, add methanol to make a solution containing 80 μg per 1 ml, and obtain it.

[0081] Emodin reference substance solution Take an appropriate amount of emodin reference substance, weigh it accurately, add methanol to make a solution containing 80 μg per 1 ml, and obtain it.

[0082] Chrysophanol reference substance solution Take an appropriate amount of chrysophanol reference substance, weigh it accurately, add methanol to make a solution containing 80 μg per 1 ml, and obtain it.

[0083] Emodin methyl ether reference substance solution Take an appropriate amount of emodin methyl ether reference ...

Embodiment 2

[0091] Embodiment 2. Preparation of Taohe Chengqi Decoction Composition

[0092] The first Taohe Chengqi Decoction composition: take the following decoction pieces by weight: 12g peach kernel, 12g rhubarb, 6g roasted licorice, 6g cassia twig, 6g mirabilite; four flavors decoction pieces (except mirabilite) are placed in a 4L casserole, Add 1400ml of water, soak for 30 minutes, cover, use the sogo heating plate as the heating device, boil (about 25min), keep a slight boil (about 85 minutes, evaporation is about 10g / min) until the decoction is about 500ml, use two layers of medical Use gauze to filter out the medicinal residues, add Glauber's salt, and boil to obtain.

[0093]The second Taohe Chengqi Decoction composition: take the following decoction pieces by weight: 1000g of peach kernels, 1000g of rhubarb, 500g of cassia twigs, 500g of Glauber's salt, and 500g of roasted licorice; Water, decocted for 1.5h to obtain the filtrate; add Glauber's salt to the filtrate, concent...

Embodiment 3

[0094] Embodiment 3. The investigation of fingerprint chromatographic conditions

[0095] (1) Selection of detection wavelength

[0096] Use octadecylsilane bonded silica gel as filler; methanol as mobile phase A, 0.1% phosphoric acid solution as mobile phase B, and carry out gradient elution as specified in the table below; flow rate 1.0ml / ml, column temperature : 30°C.

[0097] Fingerprint Mobile Phase Gradient Program

[0098]

[0099] Adopt diode array detector to carry out full-wavelength detection to need testing solution, according to the three-dimensional map of collection (see figure 1 ), and simultaneously collect the spectra at 330nm, 316nm, 278nm, 254nm, 230nm, 220nm, and 210nm wavelengths (see Figure 2-8 ), comprehensive investigation, Taohe Chengqitang granules have the largest amount of information at the wavelength of 230nm, so 230nm is finally determined to be the detection wavelength of the fingerprint of Taohe Chengqitang granules.

[0100] (2) Ins...

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Abstract

The invention discloses a method for establishing a fingerprint spectrum of Taohe Chengqi decoction composition and the fingerprint spectrum and belongs to the field of analysis of traditional Chinese medicine. According to the method, the fingerprint spectrum of the Taohe Chengqi decoction composition is established with high performance liquid chromatography, the chromatographic conditions are as follows: a chromatographic column adopts octadecylsilane chemically bonded silica as a filling agent; a detecting instrument adopts an ultraviolet detector, and detection wavelength of the fingerprint spectrum is 210-250 nm; the flow velocity is 0.9 ml / ml-1.1 ml / ml, the column temperature is 25-35 DEG C, the detection wavelength is 210-250 nm, the number of theoretical plates ought not to be lower than 3000 through calculation in accordance with a catechinic acid peak, and a flowing phase is a methanol-0.1% phosphoric acid solution which is used for performing gradient elution on a system according to a following elution sequence shown in the specification. The fingerprint spectrum reflects quality information of the Taohe Chengqi decoction comprehensively, so that the quality of the Taohe Chengqi decoction can be controlled more comprehensively and more effectively.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and in particular relates to the fingerprint of the composition of Taohe Chengqi Decoction. Background technique [0002] The composition of Taohe Chengqi Decoction is composed of peach kernel, rhubarb, licorice, cinnamon twig, and mirabilite. Symptoms such as acute cerebral hemorrhage, ovarian cyst, and endometriosis; at present, it is often added or subtracted clinically to treat the symptoms of upper, middle, and lower energies of "stasis and heat intertwined"; in Japan, this prescription ranks first in pharmacies as a Kampo medicine The sixth place, therefore, this party has a strong development value. In order to more comprehensively and effectively control the quality control level of this traditional Chinese medicine composition, and to be in line with international standards, so as to ensure the safety and efficacy of clinical medication, it is necessary to adopt more...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈世彬虢小翊刘志刚陈周全谭沛马鹏岗丁苗苗刘志东
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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