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Roselle calyx polysaccharides and preparation method and application

The technology of roselle calyx and polysaccharide is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., to achieve the effects of promoting the proliferation of spleen cells, enhancing the immune regulation function and improving the immune level.

Inactive Publication Date: 2017-03-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign researches on roselle mainly focus on the activity of roselle edible pigment and alcoholic water extract, such as the identification of pigment components, pigment stability, activity of water alcohol and its anthocyanin extract, etc. , but there are few reports on the research on roselle polysaccharides

Method used

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  • Roselle calyx polysaccharides and preparation method and application
  • Roselle calyx polysaccharides and preparation method and application
  • Roselle calyx polysaccharides and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Roselle calyx crude polysaccharide (HSP-C) was prepared from roselle calyx:

[0061] (1) The roselle calyx is crushed and passed through a 20-mesh sieve, weighed 100g, and heated under reflux with distilled water for extraction, wherein the ratio of water to material is 30:1 (mass ratio), the reflux extraction temperature is 75°C, and the reflux extraction time is 4h. The number of reflux extractions was 3 times; then the extracts were combined, centrifuged at 4000rpm for 10min, the supernatant liquid was taken, filtered through filter paper for 3 times, and the filtrate was concentrated to 200mL under reduced pressure at 40°C with a rotary evaporator to obtain a polysaccharide solution;

[0062] (2) Soak the D354FD resin in distilled water for 12 hours, then soak it in a 5% hydrochloric acid solution for 0.5 hours, wash it with water until neutral, then soak it in a 5% sodium hydroxide solution for 0.5 hours, and then wash it with distilled water To neutrality, obtain ...

Embodiment 2

[0066] Roselle calyx crude polysaccharide (HSP-C) was prepared from roselle calyx:

[0067] (1) Roselle is crushed and passed through a 20-mesh sieve, weighed 100g, and then heated and extracted with distilled water under reflux, wherein the ratio of water to material is 30:1 (mass ratio), the reflux extraction temperature is 85°C, the reflux extraction time is 3h, and the reflux extraction The number of times is 2 times; then the extracts are combined, centrifuged at 3000rpm for 8min, the supernatant liquid is taken, filtered through filter paper twice, and the filtrate is concentrated to 200mL under reduced pressure at 50°C with a rotary evaporator to obtain a polysaccharide solution;

[0068] (2) Soak the D354FD resin in distilled water for 24 hours, then soak it in 5% hydrochloric acid solution for 2 hours, wash it with distilled water until neutral, then soak it with 5% sodium hydroxide solution for 2 hours, wash it with distilled water until neutral property, obtain the ...

Embodiment 3

[0072] Roselle calyx crude polysaccharide (HSP-C) was prepared from roselle calyx:

[0073] (1) Roselle is pulverized and passed through a 20-mesh sieve, weighed 100g, and heated under reflux with distilled water for extraction, wherein the ratio of water to material is 30:1 (mass ratio), the reflux extraction temperature is 95°C, and the reflux extraction time is 2h. The number of extractions was 4 times, then the extracts were combined, centrifuged at 5000r / min for 12min, the supernatant liquid was taken, filtered 4 times with filter paper, and the filtrate was concentrated to 200mL under reduced pressure at 60°C with a rotary evaporator to obtain a polysaccharide solution;

[0074] (2) Soak the D354FD resin in distilled water for 18 hours, then soak it in 5% hydrochloric acid solution for 3 hours, wash it with distilled water until neutral, then soak it with 5% sodium hydroxide solution for 3 hours, wash it with distilled water until neutral property, obtain the pretreated ...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine and discloses roselle calyx polysaccharides and a preparation method and application. Roselle calyx coarse polysaccharide is extracted from calyx of roselle through steps of hot water reflux extraction, discoloring treatment and alcohol precipitation, and the roselle calyx coarse polysaccharide is further separated and purified through ion exchange column chromatography to obtain four refined polysaccharides. The coarse polysaccharide and the refined polysaccharides have remarkable immunoenhancement activity.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a roselle calyx polysaccharide, a preparation method and application thereof. Background technique [0002] Immunomodulatory effect is the most important bioactive function of polysaccharides, which has always been a research hotspot. The adverse reactions and side effects of immune bacterial polysaccharides and synthetic compounds have attracted widespread attention. However, most polysaccharides derived from higher plants are non-adverse substances and will not cause major side effects on the body. Therefore, polysaccharides isolated from plants have attracted great attention in biomedicine. At present, more than one hundred kinds of plant polysaccharides have been reported on the activity-related research. Studies have shown that plant polysaccharides can regulate the immune system in animals by binding to various receptors on the surface of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04
CPCA61K31/715A61K36/185C08B37/00C08B37/0003
Inventor 姜建国申春燕朱静静
Owner SOUTH CHINA UNIV OF TECH
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