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Composite antibody comprising interferon alpha/tumor antibody/graphene oxide, preparation method and application

A technology of interferon α and graphene, applied in the field of antibodies, to achieve the effect of preventing recurrence, curing tumors, shrinking and clearing tumors

Active Publication Date: 2020-01-10
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a compound antibody comprising interferon alpha / tumor antibody / graphene oxide, its preparation method and application, so as to solve the technical problem that the existing immunotherapy is only effective for a small number of patients

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  • Composite antibody comprising interferon alpha/tumor antibody/graphene oxide, preparation method and application
  • Composite antibody comprising interferon alpha/tumor antibody/graphene oxide, preparation method and application
  • Composite antibody comprising interferon alpha/tumor antibody/graphene oxide, preparation method and application

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preparation example Construction

[0042] Another aspect of the present invention provides a method for preparing a composite antibody comprising interferon alpha / tumor antibody / graphene oxide, comprising the following steps:

[0043] The interferon α and graphene oxide were mixed and reacted at 32-42°C for 2-4 hours.

[0044] Add tumor antibody and mix well, and react at 32-42°C for 4-8 hours to obtain a composite antibody comprising interferon α / tumor antibody / graphene oxide.

[0045] Specifically, 0.2 mg of GO and 2000 units of INFα can be incubated at 37° C. for 2 hours with continuous stirring. Add 1mg of tumor antibody (RTX or TRA) to the prepared 1ml INFα / GO, incubate at 37°C for 12 hours with continuous stirring, and set aside. The combination sequence of the above three substances can ensure a relatively high combination amount of the three substances, so as to ensure the therapeutic effect.

[0046] Optionally, the reaction temperature of interferon α and graphite oxide is 37°C, and the reaction tim...

Embodiment 1

[0061] GO 0.2 mg and INFα 2000 units were incubated at 37°C for 2 hours with constant agitation. The prepared 1ml INFα / GO was added with 1mg RTX monoclonal antibody, incubated at 37°C for 12 hours with continuous stirring, and set aside. GO is graphene oxide that is ultrasonically crushed and filtered through a filter with a pore size of 0.22 μm.

Embodiment 2

[0063] GO 0.1 mg and INFα 2000 units were incubated at 37°C for 2 hours with constant agitation. The prepared 1ml INFα / GO was added with 5mg TRA monoclonal antibody, incubated at 37°C for 12 hours with continuous stirring, and set aside. GO is graphene oxide that is ultrasonically crushed and filtered through a filter with a pore size of 0.22 μm.

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Abstract

The invention discloses a compound antibody containing interferon alpha / tumor antibody / oxidized graphene as well as a preparation method and applications. The compound antibody contains interferon alpha, the tumor antibody and oxidized graphene, wherein the interferon alpha, the tumor antibody and oxidized graphene are connected through non covalent bonds, and the tumor antibody is rituximab or trastuzumab. The compound antibody containing interferon alpha / tumor antibody / oxidized graphene can effectively and directly kill most tumor cells and damage the microenvironment protecting the tumor cells, and meanwhile, the interferon alpha stimulates an immune system, breaks the tumor tolerance, and promotes the specific cellular activation of tumors or the release of cell factors, so that the purposes of shrinking or eliminating tumors, preventing relapse and finally completely treating tumors are achieved.

Description

technical field [0001] The present invention relates to the field of antibodies, in particular, to a composite antibody comprising interferon alpha / tumor antibody / graphene oxide. In addition, the present invention also relates to a preparation method and application of a composite antibody comprising interferon alpha / tumor antibody / graphene oxide. Background technique [0002] Chemotherapy is a commonly used method for treating tumors. Although it has been reported in the literature that chemotherapy can reduce mortality, there are also reports in the literature that chemotherapy can neither increase the curative effect nor prolong life, because chemotherapy damages the immune system and loses the efficacy of tumor immunity. Over the past few decades, immunotherapy and targeted therapy have been two different effective strategies, each proven to have its own advantages and disadvantages. Identification of drivers of cancer by genome-guided mutations has facilitated the deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/395A61K38/21A61K47/04A61P35/00
CPCA61K38/212A61K39/39558A61K47/02A61K2300/00
Inventor 罗承科陈新建王锡阳陶立坚黄菊芳李岚邓征浩
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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