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Application of mauremys mutica polypeptide mixture and antitumor drug

An anti-tumor drug, the technology of the stone turtle, applied in the field of anti-tumor drugs and drugs, can solve the problems of poor sensitivity of external growth inhibitory signals, multi-drug resistance of tumors, and it is difficult for anti-tumor drugs to kill tumor cells. Good application prospect, the effect of inhibiting proliferation

Inactive Publication Date: 2017-03-08
深圳凯联龟业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy is one of the main means of tumor treatment. The curative effect of traditional chemotherapy is related to the patient's sensitivity to the drug, tolerance and drug toxicity. Most chemotherapy drugs are cytotoxic antineoplastic drugs with poor selectivity and toxicity. Dahe is prone to defects such as tumor multidrug resistance, and traditional chemotherapy drugs have been difficult to meet clinical needs
[0004] Due to the special growth mode of tumor cells, such as infinite proliferation, self-regulation of growth signals, poor sensitivity to external growth inhibitory signals, and strong anti-apoptotic ability, tumor cells can autonomously regulate the formation of nutrient blood vessels. And immune escape and other characteristics make it difficult for general anticancer drugs to achieve a better effect of killing tumor cells

Method used

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  • Application of mauremys mutica polypeptide mixture and antitumor drug
  • Application of mauremys mutica polypeptide mixture and antitumor drug
  • Application of mauremys mutica polypeptide mixture and antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, the preparation of stone money turtle polypeptide mixture

[0050] Mix 10 g of minced meat of the stone turtle with 80 g of ultrapure water, add neutral protease (25000 U, 37313.43 U / g) under the conditions of temperature of 45 ° C and pH of 7.5 and fully react for 10 h to obtain a reaction solution.

[0051] Inactivate the reaction solution at 85°C for 10 minutes, centrifuge at 8000r / min for 30 minutes, remove the precipitate and retain the supernatant, freeze-dry the supernatant to obtain the crude product, that is, the stone money turtle polypeptide mixture

[0052] The obtained stone money turtle polypeptide (GDP) mixture was diluted to an original concentration of 1 g / mL, and stored at 4°C for future use.

Embodiment 2

[0053] Embodiment 2, the determination of the antitumor performance of the stone money turtle polypeptide mixture

[0054] 1. Determination of inhibition of tumor cell proliferation by stone money turtle polypeptide

[0055] Take the tumor cell lines in the logarithmic growth phase to make a certain concentration of cell suspension, inoculate into 96-well plate, inoculate 5000 cells (100 μL) in each well, and place in 5% CO 2 , in a 37°C incubator, discard the original medium after culturing for 24 hours, wash it once with PBS, and then add different concentrations of the acronym tortoise peptide mixture solution, set 3 parallel wells for each concentration, and set up a control group with PBS at the same time , set 5% CO 2 , in a 37°C incubator, after incubation for 24 hours, discard the original medium, add PBS to wash 2 times, then add 10 μL MTT solution to each well, place in the incubator and incubate for 4 hours, absorb the MTT solution, and then add 100 μL DMSO solutio...

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Abstract

The invention discloses application of a mauremys mutica polypeptide mixture in the field of antitumor drug preparation and further discloses an antitumor drug which contains the mauremys mutica polypeptide mixture. The mauremys mutica polypeptide mixture has an excellent antitumor effect, and according to experimental verification, the mauremys mutica polypeptide mixture is capable of evidently inhibiting proliferation of breast cancer cells MDA-MB-231 and MCF-7, retarding a G2-M period of MDA-MB-231 and an S period of MCF-7 and evidently inhibiting proliferation of breast cancer cells MCF-7 in BALB / c-Nude mice bodies. Therefore, the mauremys mutica polypeptide mixture has a promising application prospect in the field of antitumor drug preparation.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the application of a stone money turtle polypeptide mixture in the field of preparing antitumor drugs and the antitumor medicine comprising the stone money turtle polypeptide mixture. Background technique [0002] Cancer is still a serious threat to human life and health, and global cancer cases continue to increase, largely due to population aging, world population growth, and increased carcinogenic behaviors, especially smoking in developing countries. The report mentioned that in 2015, there were 4.3 million new cancer cases in China, and more than 2.8 million cancer deaths. Some experts predict that the total number of new cancer cases in China will reach about 4 million by 2020, and the total number of cases will reach 6 million. . Lung cancer remains the most common cancer in men, accounting for 17% of overall cancer incidence and 14% of mortality. Breast cancer is the most commo...

Claims

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Application Information

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IPC IPC(8): A61K38/02A61P35/00A61K35/586
CPCA61K35/586A61K38/02
Inventor 单凯
Owner 深圳凯联龟业有限公司
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