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Chicken salmonella enteritidis infection resistance molecular marker Wnt7b detection method and application thereof

A Salmonella Enteritidis, molecular marker technology, applied in the field of genetic engineering

Active Publication Date: 2017-02-22
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rs313644723 locus is located in the upstream region of the Wnt7b gene, but no studies have been conducted on the genotyping of this locus and its association with resistance to Salmonella Enteritidis infection

Method used

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  • Chicken salmonella enteritidis infection resistance molecular marker Wnt7b detection method and application thereof
  • Chicken salmonella enteritidis infection resistance molecular marker Wnt7b detection method and application thereof
  • Chicken salmonella enteritidis infection resistance molecular marker Wnt7b detection method and application thereof

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Embodiment 1

[0025] Acquisition of chickens to be tested

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Abstract

The invention relates to the technical field of gene engineering, in particular to a chicken salmonella enteritidis infection resistance molecular marker Wnt7b detection method and application thereof. A chicken Wnt7b gene rs313644723 site genotyping specific primer and genotyping PCR (polymerase chain reaction) conditions matched with the primer are provided herein, salmonella enteritidis infection resistance related site genotypes can be accurately detected by using the primer and the method, and theoretical basis and scientific basis are provided for screening and disease-resistant breeding of chicken salmonella enteritidis infection resistant individuals.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, and provides a detection method of Salmonella enteritidis infection resistance molecular marker Wnt7b and an application thereof. Background technique [0002] Salmonella enteritidis is a Gram-negative bacillus with more than 2,500 serotypes. It can not only cause disease in livestock and poultry, but also cause serious economic losses in the breeding industry. It is also one of the main pathogens of food-borne diseases. It is one of the most frequently reported pathogenic microorganisms. Relevant reports pointed out that during 2004, 19,000 people per 1 million people in the United States were hospitalized due to Salmonella infection, and as many as 300 people died due to Salmonella infection; Disease outbreaks. [0003] Poultry and its related processed products are the main source of infection of Salmonella Enteritidis, which can enter egg products through eggshells and can repro...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172Y02A50/30
Inventor 李显耀薛念国王莎莎吴桂贤
Owner SHANDONG AGRICULTURAL UNIVERSITY
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