Fat filler and preparation method thereof

Abstract

The invention discloses an autologous fat filler and a preparation method thereof, wherein the autologous fat filler is constructed by simulating a tissue engineering method. The autologous fat filler comprises the following ingredients of a cytoskeleton, a cellular constituent, a growth factor and a scar resistant factor at the volume ratio of (109-115):(15-30):4:0.5. The effective ingredients are combined, the cytoskeleton part provides a skeleton made of a plant cell ingredient, an injectable gel is favorable for the adhesion of a cell, a washing fat occupies most of a filling volume, a stem cell and an SVF (Stromal Vascular Fraction) component are differentiated into an adipose cell and a vascular endothelial cell, and various excreted factors are good for maintaining a transplanting microenvironment and generating a blood vessel. According to the provided autologous fat filler and the preparation method thereof, the stability of the transplanting fat is effectively improved, the absorption of the transplanted fat is reduced, the untoward effect of fibrosis of a transplant is reduced, and the development of an autologous fat face filling plasty is promoted.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a fat filler and a preparation method thereof. Background technique [0002] Various congenital and traumatic soft tissue defects have always been one of the most difficult problems for plastic surgeons. The causes of soft tissue defects include severe burns, infections, various traumas, and congenital diseases. For soft tissue loss, the commonly used treatment Methods include skin flap transplantation, prosthetic implantation, soft tissue filling, etc. Among them, soft tissue filling has the advantages of less trauma, good effect, and strong plasticity. The commonly used fillers in clinical practice include silica gel, collagen, and sodium hyaluronate, but there are disadvantages of rejection or unsustainable postoperative curative effect. [0003] Adipose tissue opens up new directions for the choice of soft tissue fillers. Fat has the advantages of good biocompatibilit...

AI Technical Summary

Problems solved by technology

However, the content of growth factors in fat transplantation is limited, and the half-life of exogenously added VEGF factors is short, less than 1 hour under normal physiological conditions in vivo.

Some people have considered using VEGF gene transfection and adding a slow-release system, but the controllability of the transfection method is poor, and the slow-release system is easy to bring in impurities; the above two methods are poor in clinical operability

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