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Wheat hybridization method for increasing setting rate

A hybridization method and a technology of seed setting rate, which are applied in the field of wheat hybridization, can solve the problems of cumbersome sowing operations, affecting the success rate of pollination, affecting the efficiency and yield of hybrid breeding, etc., to achieve easy control, improve effectiveness and efficiency, and avoid false hybridization Effect

Inactive Publication Date: 2017-02-22
漯河市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Then in the whole process of hybridization, on the one hand, the method of artificial pollination will affect the success rate of pollination, and then affect the seed setting rate of hybrid varieties; Nutrient supplementation and growth status at the post-filling stage affect the seed-setting rate and grain weight of wheat to a certain extent, which in turn affects the efficiency and yield of cross-breeding
[0003] In the process of crossing parents of different varieties of wheat, in order to avoid false hybridization, parchment paper bags are usually used to isolate the selected female and male ears, and then artificial pollination is carried out by twisting method or anther smearing method. This method of hybridization requires the male and female parents to be planted in one area adjacent to each other, and the sowing operation is cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A wheat hybridization method for improving seed setting rate, comprising the following steps:

[0024] 1) Sow the male parent and the female parent, and when the male parent and the female parent grow to the flower bud differentiation stage, spray the wheat growth-promoting agent on the male parent and the female parent;

[0025] 2) When the male parent and the female parent grow to the heading stage, when the female parent ear is completely drawn out, use a pin to peel off the flag leaf and cut off 1 / 3 of each spikelet, remove the stamen, and spray the pistil growth promoter, Get the cellophane bag and cover the female parent's ear after the treatment in the cellophane bag, and fix the test tube filled with water at 5-10 cm above the female parent's ear in the cellophane bag with iron wire, when the selected male parent hybridized with the female parent grows to During the flowering stage, intercept the male parent ear and put it into the fixed water test tube in the c...

Embodiment 2

[0032] This embodiment improves the wheat hybridization method of the seed setting rate. The difference from the embodiment is that the wheat growth-stimulating agent is prepared from the following raw materials in percentage by weight: amino acid chelated zinc 0.1%, amino acid chelated calcium 0.1%, amino acid Chelated boron 0.1%, superphosphate 0.2%, ammonium phytate 0.2%, nucleotide 0.3%, rice vinegar 1%, the balance is water;

[0033] The pistil growth-promoting agent is prepared from the following raw materials in percentage by weight: amino acid chelated boron 0.02%, amino acid chelated calcium 0.4%, bovine serum albumin 1%, mannitol 5%, pectinase 0.04%, Cellulase 0.1%, hemicellulase 0.1%, gibberellic acid 0.05%, lansoprazole 0.05%, sodium nitroprusside 0.1%, the balance is water;

[0034] The grouting regulator is prepared from the following raw materials in weight percent: gibberellin 0.05%, potassium dihydrogen phosphate 0.1%, humic acid 0.05%, amine fresh fat DA-6 0....

Embodiment 3

[0037] This embodiment improves the wheat hybridization method of the seed setting rate, and the difference from the embodiment is that the wheat growth-stimulating agent is prepared from the following raw materials in percentage by weight: amino acid chelated zinc 0.2%, amino acid chelated calcium 0.2%, amino acid Chelated boron 0.2%, superphosphate 0.5%, ammonium phytate 0.4%, nucleotide 0.5%, rice vinegar 2%, the balance is water;

[0038] The pistil growth-promoting agent is prepared from the following raw materials in percentage by weight: amino acid chelated boron 0.06%, amino acid chelated calcium 0.6%, bovine serum albumin 2%, mannitol 6%, pectinase 0.2%, Cellulase 0.3%, hemicellulase 0.3%, gibberellic acid 0.08%, lansoprazole 0.06%, sodium nitroprusside 0.2%, the balance is water;

[0039] The grouting stage regulator is prepared from the following raw materials in percentage by weight: gibberellin 0.08%, potassium dihydrogen phosphate 0.2%, humic acid 0.07%, amine fr...

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Abstract

The invention relates to the technical field of hybrid breeding, in particular to a wheat hybridization method for increasing the setting rate. The method comprises the steps that when wheat grows to a flower bud differentiation period, a wheat growth promoter is sprayed to male parents and female parents; after female parent ears are completely headed, flag leaves are stripped off, one third of each spikelet is cut off, stamens are removed, a pistil growth promoter is sprayed, glassine bags are taken, the female parent ears are covered with the glassine bags, test tubes containing water are fixed in the glassine bags, male parent ears are cut out to be placed in the test tubes which are fixed in the glassine bags and contain water when the selected male parents hybridized with the female parents grow to a flowering period, the glassine bags are shaken in a hybridizing pollination period to complete natural pollination between the male parents and the female parents, and after pollination is completed, the glassine bags and the test tubes are taken down; when the pollinated female parents grow to a filling period, a filling period regulator is sprayed. According to the method, the pollination method is combined with nutrient supply of wheat in different periods, false hybridization is avoided, the hybrid breeding efficiency is improved, and the setting rate and the yield are increased.

Description

technical field [0001] The invention relates to the technical field of hybrid breeding, in particular to a wheat hybridization method for improving seed setting rate. Background technique [0002] Wheat is the food crop with the largest total output and high nutritional value in the world. With the further increase of population, the area of ​​cultivated land per capita is decreasing year by year. Food problem is one of the most important problems we are facing at present. Heterosis is a common phenomenon in the biological world. In the existing wheat hybridization process, the parents are generally planted separately. When the male and female parents reach the flowering stage, artificial pollination is carried out. After the pollination is successful, the ears grow to maturity. Then in the whole process of hybridization, on the one hand, the method of artificial pollination will affect the success rate of pollination, and then affect the seed setting rate of hybrid varieti...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06A01H1/02C05G3/00C05G3/02C05G3/04C05G3/60C05G3/80
CPCA01G7/06A01G22/00A01H1/02C05B1/02C05B7/00C05C11/00C05G3/60C05G3/80C05G5/23C05C3/00C05D3/00C05D9/02C05F11/00C05F11/08C05F11/02
Inventor 郭春强曹燕燕廖平安葛昌斌李亚黄杰乔冀良胡书莲张中州于蕾
Owner 漯河市农业科学院
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